公开(公告)号：US10266820B2

公开(公告)日：2019-04-23

申请号：US14754556

申请日：2015-06-29

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Hyung Jun Ahn ,  Ick Chan Kwon ,  Mihue Jang ,  Jong Hwan Kim

IPC: C12N15/11

Abstract: A RNA/DNA nanoparticle for delivering siRNA where a RNA transcript including at least one hairpin structure hybridizes DNA-cholesterol conjugate and folate-DNA conjugate including a complementary sequence to the RNA transcript, and a composition including the RNA/DNA nanoparticle is provided. More specifically, because various siRNA used for different applications can be contained in the RNA/DNA nanoparticle for delivering siRNA at a high loading efficiency, and has stability to the outer attacks such as nuclease degradation. The RNA/DNA nanoparticle siRNA can be prepared by self-assembly without using polycationic agent which is harmful agent for body. The folate targeting to various cancer cells can accumulate the nanoparticle selectively on target cancer cell after intravenous injection, and make excellent gene-silencing effect inside the cancer tissue, thereby being used as a good agent for treating cancers.

公开(公告)号：US20240151728A1

公开(公告)日：2024-05-09

申请号：US18487614

申请日：2023-10-16

Applicant: Korea Institute of Science and Technology ,  SEOUL NATIONAL UNIVERSITY R&db FOUNDATION

Inventor： Jihye SEONG ,  Mihue Jang ,  Haenim Lee ,  Hyejin Yoo ,  Chang-Han Lee ,  Jiyun Jeong ,  Hang-Rae Kim ,  Soojin Lee

IPC: G01N33/574 ,  G01N33/533 ,  G01N33/542 ,  G01N33/68

CPC classification number: G01N33/57492 ,  G01N33/533 ,  G01N33/542 ,  G01N33/6845 ,  C12N2503/00

Abstract: The disclosure relates to a fluorescence resonance energy transfer (FRET)-based biosensor for sensing chimeric antigen receptor (CAR) activity and use thereof, and in particular, to a FRET-based biosensor, which simultaneously detects binding of an antigen-binding receptor domain to a cancer antigen and consequent activation of T cells, and a method of screening for CAR activity in a live cell by using the FRET-based biosensor.

公开(公告)号：US11649282B2

公开(公告)日：2023-05-16

申请号：US16771918

申请日：2020-03-10

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY ,  THE ASAN FOUNDATION ,  UNIVERSITY OF ULSAN FOUNDATION FOR INDUSTRY COOPERATION

Inventor： Mihue Jang ,  Eunsung Jun ,  Anna Ju

IPC: C07K16/28 ,  A61K35/17 ,  C07K14/705 ,  C12N15/86 ,  A61P35/00 ,  C07K14/725 ,  C12N5/0783 ,  A61K38/00 ,  A61K39/00

CPC classification number: C07K16/28 ,  A61K35/17 ,  A61P35/00 ,  C07K14/7051 ,  C07K14/70575 ,  C07K14/70578 ,  C12N5/0646 ,  C12N15/86 ,  A61K38/00 ,  A61K2039/505 ,  A61K2039/5154 ,  A61K2039/5156 ,  C07K2317/622 ,  C07K2317/73 ,  C07K2317/76 ,  C07K2319/02 ,  C07K2319/30 ,  C07K2319/33 ,  C12N2510/00 ,  C12N2740/15043

Abstract: Provided are a genetically modified immunocyte expressing a chimeric antigen receptor (CAR) comprising an antigen binding domain specifically binding to cancer cells and/or expressing TRAIL, a composition for preventing or treating cancer, the composition comprising the immunocytes, a cell therapeutic agent, a method of providing information for cancer diagnosis, and a method of preparing the genetically modified immunocyte.