公开(公告)号：US12117457B2

公开(公告)日：2024-10-15

申请号：US17219023

申请日：2021-03-31

Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

Inventor： Junghyun Son ,  Yoeseph Cho ,  Oh-Seung Kwon ,  Hana Park ,  Jin Woo Park ,  Yejin Lee ,  Seongeun Jeon

IPC: G01N33/94

CPC classification number: G01N33/9406 ,  G01N33/9413 ,  G01N33/942 ,  G01N33/9426 ,  G01N33/944

Abstract: Disclosed is a method for simultaneous analysis of neurotransmitters and/or their metabolites. The method includes (a) separating analytes including a plurality of neurotransmitters and/or their metabolites from a sample selected from body tissues, body fluids, secretions, and excretions, (b) derivatizing the analytes with ethyl chloroformate to obtain derivatives of the plurality of neurotransmitters and/or their metabolites, (c) separating the derivatives of the plurality of neurotransmitters and/or their metabolites by liquid chromatography, and (d) subjecting the separated derivatives of the neurotransmitters or their metabolites to multiple reaction monitoring (MRM) using a mass spectrometer. According to the method, a plurality of neurotransmitters in a very small amount of sample can be simultaneously analyzed in an accurate and rapid manner based on derivatization to increase the stability and ionization efficiency of the substances.