公开(公告)号：US20220089648A1

公开(公告)日：2022-03-24

申请号：US17229355

申请日：2021-04-13

申请人： KUROS BIOSCIENCES AG

发明人： Marcel EMMERLING ,  Frank HENNECKE ,  Holger PFRÜNDER ,  Martin RHIEL ,  Philipp STEINER

IPC分类号： C07K14/005 ,  C12N7/00

摘要： This invention provides a robust fermentation process for the expression of a capsid protein of a bacteriophage which is forming a VLP by self-assembly, wherein the process is scalable to a commercial production scale and wherein the expression rate of the capsid protein is controlled to obtain improved yield of soluble capsid protein. This is achieved by combining the advantages of fed-batch culture and of lactose induced expression systems with specific process parameters providing improved repression of the promoter during the growth phase and high plasmid retention throughout the process.

公开(公告)号：US20200223891A1

公开(公告)日：2020-07-16

申请号：US16654232

申请日：2019-10-16

申请人： KUROS BIOSCIENCES AG

发明人： Marcel EMMERLING ,  Frank HENNECKE ,  Holger PFRÜNDER ,  Martin RHIEL ,  Philipp STEINER

IPC分类号： C07K14/005 ,  C12N7/00

摘要： This invention provides a robust fermentation process for the expression of a capsid protein of a bacteriophage which is forming a VLP by self-assembly, wherein the process is scalable to a commercial production scale and wherein the expression rate of the capsid protein is controlled to obtain improved yield of soluble capsid protein. This is achieved by combining the advantages of fed-batch culture and of lactose induced expression systems with specific process parameters providing improved repression of the promoter during the growth phase and high plasmid retention throughout the process.

公开(公告)号：US20170305972A1

公开(公告)日：2017-10-26

申请号：US15353884

申请日：2016-11-17

申请人： KUROS BIOSCIENCES AG

发明人： Marcel EMMERLING ,  Frank HENNECKE ,  Holger PFRÜNDER ,  Martin RHIEL ,  Philipp STEINER

IPC分类号： C07K14/005 ,  C12N7/00 ,  A61K39/00

摘要： This invention provides a robust fermentation process for the expression of a capsid protein of a bacteriophage which is forming a VLP by self-assembly, wherein the process is scalable to a commercial production scale and wherein the expression rate of the capsid protein is controlled to obtain improved yield of soluble capsid protein. This is achieved by combining the advantages of fed-batch culture and of lactose induced expression systems with specific process parameters providing improved repression of the promoter during the growth phase and high plasmid retention throughout the process.