公开(公告)号：US20210309960A1

公开(公告)日：2021-10-07

申请号：US17266984

申请日：2019-08-09

申请人： KYOWA HAKKO BIO CO., LTD.

发明人： Tohru DAIRI ,  Yasuharu SATOH ,  Shohei HAYASHI ,  Mai NAKA ,  Tetsuro UJIHARA

IPC分类号： C12N1/10 ,  C12N15/70 ,  C12P7/64

摘要： An object of the present invention is to provide a microorganism that efficiently produces EPA and a method for producing EPA using the microorganism. The present invention relates to a microorganism having an ability to produce docosahexaenoic acid (DHA), wherein the microorganism contains a protein composed of an amino acid sequence in which at least one of the amino acid residues at positions 6, 65, 230, 231, and 275 in the amino acid sequence represented by SEQ ID NO: 2 has been substituted with another amino acid residue (mutated OrfB), and is capable of producing eicosapentaenoic acid (EPA), and the like.

公开(公告)号：US20210309987A1

公开(公告)日：2021-10-07

申请号：US17266976

申请日：2019-08-09

申请人： KYOWA HAKKO BIO CO., LTD.

发明人： Tohru DAIRI ,  Yasuharu SATOH ,  Shohei HAYASHI ,  Yasushi OGASAWARA ,  Tetsuro UJIHARA

IPC分类号： C12N9/00 ,  C12P7/64 ,  C12N9/10 ,  C12N9/02 ,  C12N9/12

摘要： An object of the present invention is to provide a microorganism that efficiently produces a PUFA and a method for producing a PUFA using the microorganism. The present invention relates to a microorganism capable of producing a polyunsaturated fatty acid (PUFA), in which a gene encoding an exogenous polyketide synthase dehydratase (PS-DH) domain having a higher activity against 3-hydroxyhexanoyl acyl carrier protein (3-hydroxyhexanoyl ACP) than an endogenous FabA-like β-hydroxyacyl-ACP dehydratase (FabA-DH) domain has been introduced into a microorganism having a PUFA metabolic pathway, and the like.

公开(公告)号：US20230235271A1

公开(公告)日：2023-07-27

申请号：US18169487

申请日：2023-02-15

申请人： KYOWA HAKKO BIO CO., LTD.

发明人： Tohru DAIRI ,  Yasuharu SATOH ,  Shohei HAYASHI ,  Mai NAKA ,  Tetsuro UJIHARA

IPC分类号： C12N1/10 ,  C12N15/70 ,  C12P7/64

CPC分类号： C12N1/105 ,  C12N15/70 ,  C12P7/64 ,  C12R2001/90

摘要： An object of the present invention is to provide a microorganism that efficiently produces EPA and a method for producing EPA using the microorganism. The present invention relates to a microorganism having an ability to produce docosahexaenoic acid (DHA), wherein the microorganism contains a protein composed of an amino acid sequence in which at least one of the amino acid residues at positions 6, 65, 230, 231, and 275 in the amino acid sequence represented by SEQ ID NO: 2 has been substituted with another amino acid residue (mutated OrfB), and is capable of producing eicosapentaenoic acid (EPA), and the like.