公开(公告)号：US20130053594A1

公开(公告)日：2013-02-28

申请号：US13516079

申请日：2010-12-17

申请人： Ka Yee Christina Lee ,  Niels Holten-Andersen ,  J. Herbert Waite

发明人： Ka Yee Christina Lee ,  Niels Holten-Andersen ,  J. Herbert Waite

IPC分类号： C07F15/02 ,  C07F7/28 ,  C07F5/06

CPC分类号： C08G65/3317 ,  C08G65/329 ,  C08G65/33396

摘要： Methods for making self-healing polymer and gel compositions with crosslinks comprising coordinate bonds between monomer subunits and metals are disclosed.

摘要翻译： 公开了制备具有包含单体亚基和金属之间的配位键的交联的自愈合聚合物和凝胶组合物的方法。

公开(公告)号：US4496397A

公开(公告)日：1985-01-29

申请号：US587181

申请日：1984-03-07

申请人： J. Herbert Waite

发明人： J. Herbert Waite

IPC分类号： A23J1/04 ,  C09J189/00 ,  C07G7/00 ,  C08L89/00 ,  C09J3/20

CPC分类号： C09J189/00 ,  A23J1/04 ,  Y10S530/857

摘要： A process is provided for purifying and stabilizing marine mussel polyphenolic proteins rich in 3,4-dihydroxyphenylalanine (dopa) and hydroxyproline (hyp) while obtaining high yields thereof. The process includes the steps of providing an acid soluble extract of the dopa-containing proteins, removing the low molecular weight material from the extract and reacting the remaining proteinaceous material with a water soluble borate at a pH of 7.0-9.0 to provide a soluble borate complex of the dopa-containing protein while precipitating impurities. The complex is separated from the precipitate and may be concentrated for storage or treated with an acetic acid solution and either concentrated or lyophilized and stored under an inert atmosphere. The purified proteins exhibit a dopa:protein index of purity ratio of at least about 0.10.

摘要翻译： 提供了一种纯化和稳定富含3,4-二羟基苯丙氨酸（多巴）和羟脯氨酸（hyp）的海洋贻贝多酚蛋白的方法，同时获得高产率。 该方法包括以下步骤：提供含多巴蛋白质的酸溶性提取物，从提取物中除去低分子量物质，并将剩余的蛋白质物质与pH9.0-9.0的水溶性硼酸盐反应，得到可溶性硼酸盐 含多巴的蛋白质的复合物同时沉淀杂质。 将复合物与沉淀物分离，并且可以浓缩用于储存或用乙酸溶液处理，并将其浓缩或冻干并在惰性气氛下储存。 纯化的蛋白质显示出多巴：蛋白质的纯度比指数至少约为0.10。

公开(公告)号：US5574134A

公开(公告)日：1996-11-12

申请号：US839745

申请日：1992-02-19

申请人： J. Herbert Waite

发明人： J. Herbert Waite

IPC分类号： C07K14/435 ,  A61K38/04

CPC分类号： C07K14/43504

摘要： A water-resistant polyphenolic protein adhesive has been obtained from the Atlantic ribbed mussel, Geukensia (Modiolus) demissa. This protein provides a natural model for synthetic or bioengineered adhesives and is characterized by a Gly-Tyr-Lys or, more frequently, Gly-Dop-Lys "tail" fragment of a repeating octapeptide or nonapeptide unit. A complete octapeptide or nonapeptide unit (including both the 5- or 6-amino acid residue "head" as well as the 3-residue "tail") can be represented as follows:[Gln]Glu].sub.x -Thr/Ala-Gly-Dop/Tyr-Y.sup.1 -Y.sup.2 -Gly-Tyr/Dop-Lyswhere x is zero or one,Y.sup.1 is Val, Leu, Asp or Ser, andY.sup.2 is Ala, Pro, Hyp, or Leu.The tripeptide "tail" and/or the 5- or 6-residue "head" of the polypeptide is useful as a single unit (curable with difunctional crosslinkers or the like) or as a repeating unit.

摘要翻译： 已经从大西洋罗纹贻贝（Geusensia（Modiolus）demissa）获得了耐水多酚蛋白粘合剂。 该蛋白提供了合成或生物工程化粘合剂的天然模型，其特征在于重复八肽或单肽单元的Gly-Tyr-Lys或更频繁的Gly-Dop-Lys“尾”片段。 完整的八肽或非肽单元（包括5-或6-氨基酸残基“头”以及3-残基“尾”）可以表示如下：[Gln] Glu] x-Thr / Ala-Gly -Dop / Tyr-Y1-Y2-Gly-Tyr / Dop-Lys，其中x为0或1，Y1为Val，Leu，Asp或Ser，Y2为Ala，Pro，Hyp或Leu。 多肽“尾”和/或该多肽的5-或6-残基“头”可用作单一单元（可用双功能交联剂等固化）或作为重复单元。

公开(公告)号：US4808702A

公开(公告)日：1989-02-28

申请号：US55450

申请日：1987-06-01

申请人： J. Herbert Waite

发明人： J. Herbert Waite

IPC分类号： A61K38/00 ,  C07K14/435 ,  C07C103/52 ,  C12P21/06

CPC分类号： C07K14/435 ,  A61K38/00 ,  Y10S930/29

摘要： Methods are described for the preparation and isolation of novel decapeptides of the formula: ##STR1## wherein each X is independently selected from the group comprising hydroxyl and hydrogen, wherein each R is independently selected from the group comprising hydrogen and methyl, from bioadhesive polyphenolic proteins which comprise: ##STR2## wherein n is a whole number from about 60 to about 100, wherein each X is independently selected from the group comprising hydroxyl and hydrogen, and wherein each R is independently sselected from the group comprising hydrogen and methyl.Such decapeptides may be used to construct large polyphenolic molecules comprising from about 1 to about 1000 decapeptide repeating units and wherein the linking group is selected from the group comprising amino acid, oligopeptide and bifunctional spacer.

摘要翻译： 描述了用于制备和分离下式的新型十肽的方法：其中每个X独立地选自羟基和氢，其中每个R独立地选自包括以下的基团： 氢和甲基，来自生物粘附多酚蛋白，其包含：其中n为约60至约100的整数，其中每个X独立地选自羟基和氢，以及 其中每个R独立地选自氢和甲基的基团。 这种十肽可用于构建包含约1至约1000个十肽重复单元的大多酚分子，并且其中该连接基团选自氨基酸，寡肽和双功能间隔基。

公开(公告)号：US4687740A

公开(公告)日：1987-08-18

申请号：US820143

申请日：1986-01-21

申请人： J. Herbert Waite

发明人： J. Herbert Waite

IPC分类号： A61K38/00 ,  C07K14/435 ,  C12P21/06

CPC分类号： C07K14/435 ,  A61K38/00

摘要： Methods are described for the preparation and isolation of novel decapeptides of the formula: ##STR1## wherein each X is independently selected from the group comprising hydroxyl and hydrogen, wherein each R is independently selected from the group containing hydrogen and methyl, from bioadhesive polyphenolic proteins which comprise: ##STR2## wherein n is a whole number from about 60 to about 100, wherein each X is independently selected from the group comprising hydroxyl and hydrogen, and wherein each R is independently selected from the group comprising hydrogen and methyl.Such decapeptides may be used to construct large polyphenolic molecules comprising from about 1 to about 1000 decapeptide repeating units and wherein the linking group is selected from the group comprising amino acid, oligopeptide and bifunctional spacer.

摘要翻译： 描述了用于制备和分离下式的新型十肽的方法：其中每个X独立地选自羟基和氢，其中每个R独立地选自含有（C 1 -C 6） 氢和甲基，来自生物粘附多酚蛋白，其包含：其中n为约60至约100的整数，其中每个X独立地选自羟基和氢，以及 其中每个R独立地选自氢和甲基。 这种十肽可用于构建包含约1至约1000个十肽重复单元的大多酚分子，并且其中该连接基团选自氨基酸，寡肽和双功能间隔基。

公开(公告)号：US4585585A

公开(公告)日：1986-04-29

申请号：US587132

申请日：1984-03-07

申请人： J. Herbert Waite

发明人： J. Herbert Waite

IPC分类号： A61K38/00 ,  C07K14/435 ,  C12P21/06

CPC分类号： C07K14/435 ,  A61K38/00

摘要： Methods are described for the preparation and isolation of novel decapeptides of the formula: ##STR1## wherein each X is independently selected from the group comprising hydroxyl and hydrogen, wherein each R is independently selected from the group comprising hydrogen and methyl, from bioadhesive polyphenolic proteins which comprise: ##STR2## wherein n is a whole number from about 60 to about 100, wherein each X is independently selected from the group comprising hydroxyl and hydrogen, and wherein each R is independently selected from the group comprising hydrogen and methyl.Such decapeptides may be used to construct large polyphenolic molecules comprising from about 1 to about 1000 decapeptide repeating units and wherein the linking group is selected from the group comprising amino acid, oligopeptide and bifunctional spacer.

摘要翻译： 描述了用于制备和分离下式的新型十肽的方法：其中每个X独立地选自羟基和氢，其中每个R独立地选自包括以下的基团： 氢和甲基，来自生物粘附多酚蛋白，其包含：其中n为约60至约100的整数，其中每个X独立地选自羟基和氢，以及 其中每个R独立地选自氢和甲基。 这种十肽可用于构建包含约1至约1000个十肽重复单元的大多酚分子，并且其中该连接基团选自氨基酸，寡肽和双功能间隔基。