公开(公告)号：US20070010439A1

公开(公告)日：2007-01-11

申请号：US11247585

申请日：2005-10-12

申请人： Kaare Gautvik ,  Peter Alestrom ,  Tordis Oyen ,  Odd Gabrielsen

发明人： Kaare Gautvik ,  Peter Alestrom ,  Tordis Oyen ,  Odd Gabrielsen

IPC分类号： A61K38/29 ,  C12N1/18 ,  C07K14/635

CPC分类号： C07K14/635 ,  A61K38/00 ,  C07K2319/02 ,  C12N15/64 ,  C12N15/66 ,  C12N15/67 ,  C12N15/70 ,  C12N15/81

摘要： The invention provides recombinant plasmids containing in DNA sequences coding for human preproparathyroid hormone. The invention further provides microorganisms, for example E. coli, transformed by these plasmids. The invention also provides a plasmid for insertion into yeast and a transformed yeast in which the plasmid contains DNA coding for parathyroid hormone. Parathyroid hormone is then secreted by the transformed yeast. Further the invention provides alternate polypeptides having parathyroid hormone activity, including PTH analogs, fragments and extensions, and provides alternate leader sequences and secretion signal sequences which can be used in the present invention. Finally, there are provided methods for purification of the secreted PTH hormone and/or derivatives.

摘要翻译： 本发明提供含有编码人前甲状腺激素的DNA序列的重组质粒。 本发明还提供了由这些质粒转化的微生物，例如大肠杆菌。 本发明还提供了用于插入酵母的质粒和其中质粒含有编码甲状旁腺激素的DNA的转化酵母。 甲状旁腺激素然后被转化的酵母分泌。 此外，本发明提供了具有甲状旁腺激素活性的替代多肽，包括PTH类似物，片段和延伸，并提供可用于本发明的替代前导序列和分泌信号序列。 最后，提供了分泌的PTH激素和/或衍生物的纯化方法。