-
公开(公告)号:US07118867B2
公开(公告)日:2006-10-10
申请号:US10300576
申请日:2002-11-19
申请人: Karim Tabiti , Gisela Betzl , Richie Soong , Randy P. Rasmussen , Deepika Marine Desilva , John G. Ward , Hallegh Page Millward
发明人: Karim Tabiti , Gisela Betzl , Richie Soong , Randy P. Rasmussen , Deepika Marine Desilva , John G. Ward , Hallegh Page Millward
CPC分类号: C12Q1/48 , C12Q1/6851 , C12Q1/686 , G01N2333/9125 , C12Q2561/113 , C12Q2545/114 , C12Q2537/143 , C12Q2527/127
摘要: The invention provides a method for quantitative multiplex PCR of two targets whereby one target is present in at least 100-fold molar excess over that of the other target. The method further comprises the addition of a thermostable DNA polymerase with a final concentration of at least 0.5 units/μl.
摘要翻译: 本发明提供了一种用于两个靶的定量多重PCR的方法,其中一个靶以比另一个靶的至少100倍摩尔过量存在。 该方法还包括加入终浓度至少为0.5单位/毫升的热稳定DNA聚合酶。