公开(公告)号：US20180274034A1

公开(公告)日：2018-09-27

申请号：US15947473

申请日：2018-04-06

申请人： Avellino Co., Ltd.

发明人： Gene Lee ,  Jung Kuk Yun

IPC分类号： C12Q1/6883 ,  C12Q1/686

CPC分类号： C12Q1/6883 ,  C12Q1/686 ,  C12Q2600/112 ,  C12Q2600/156 ,  C12Q2561/113

摘要： The present invention relates to a real-time PCR primer pair and probe for diagnosing Avellino corneal dystrophy, and more particularly to a real-time PCR primer pair and probe for diagnosing Avellino corneal dystrophy, which can accurately diagnose the presence or absence of a mutation in exon 4 of BIGH3 gene, which is responsible for Avellino corneal dystrophy. The use of the primer pair and probe according to the invention can diagnose Avellino corneal dystrophy in a more rapid and accurate manner than a conventional method that uses a DNA chip or PCR.

公开(公告)号：US10077475B2

公开(公告)日：2018-09-18

申请号：US14633094

申请日：2015-02-26

申请人： CALIFORNIA INSTITUTE OF TECHNOLOGY

发明人： Emil P. Kartalov ,  Aditya Rajagopal ,  Axel Scherer ,  Mark D. Goldberg

IPC分类号： C12Q1/68 ,  C12Q1/6883 ,  C12Q1/6851 ,  C12Q1/70

CPC分类号： C12Q1/6883 ,  C12Q1/68 ,  C12Q1/6851 ,  C12Q1/701 ,  C12Q1/703 ,  C12Q2600/156 ,  Y10T436/143333 ,  C12Q2561/113 ,  C12Q2565/101 ,  C12Q2527/101 ,  C12Q2565/1015 ,  C12Q2565/133

摘要： FRET-based analytes detection and related methods and systems are described where a pair of FRET labeled primers and/or oligonucleotides are used that are specific for target sequences located at a distance up to four time the Förster distance of the FRET chromophores presented on the FRET labeled primers and/or oligonucleotides one with respect to the other in one or more polynucleotide analyte; in particular the pair of FRET labeled primers and/or oligonucleotides is combined with a sample and subjected to one or more polynucleotide amplification reactions before measuring FRET signals from at least one FRET chromophore.

公开(公告)号：US20180243741A1

公开(公告)日：2018-08-30

申请号：US15829665

申请日：2017-12-01

申请人： Fluidigm Corporation

发明人： Marc A. Unger ,  Lincoln McBride ,  Geoffrey Facer

IPC分类号： B01L3/00 ,  C12Q1/686 ,  G01N27/453 ,  B01L7/00

CPC分类号： B01L3/502738 ,  B01L7/52 ,  B01L2200/147 ,  B01L2300/0636 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/0887 ,  B01L2300/0893 ,  B01L2300/123 ,  B01L2300/1805 ,  B01L2300/1827 ,  B01L2400/0481 ,  B01L2400/0655 ,  C12Q1/6848 ,  C12Q1/6851 ,  C12Q1/686 ,  C12Q2565/629 ,  G01N27/44791 ,  G01N27/453 ,  C12Q2561/113 ,  C12Q2563/159 ,  C12Q2531/113

摘要： Devices and methods for performing the relative concentration of a target in a sample, the sample containing both target and non-target components, the method performed by partitioning the sample into a large number of reaction volumes such that the target is concentrated relative to the non-target, and performing a detection assay upon each reaction volume to detect the target.

公开(公告)号：US20180187245A1

公开(公告)日：2018-07-05

申请号：US15851383

申请日：2017-12-21

申请人： Omniome, Inc.

发明人： Corey M. Dambacher ,  Devon Cayer ,  Richard LeCoultre ,  Joseph Rokicki ,  Kerry Wilson ,  Eugene Tu ,  Kandaswamy Vijayan

IPC分类号： C12Q1/6816

CPC分类号： C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q2521/101 ,  C12Q2527/125 ,  C12Q2537/143 ,  C12Q2537/149 ,  C12Q2561/113 ,  C12Q2563/107 ,  C12Q2563/125 ,  C12Q2565/629 ,  C12Q2535/125

摘要： Method of identifying a cognate nucleotide (i.e., the “next correct nucleotide”) for a primed template nucleic acid molecule. In some embodiments, an ordered or random array of primed target nucleic acids characterized by different cognate nucleotides can be evaluated using a single imaging step to identify different cognate nucleotides for a collection of different primed template nucleic acid molecules. An optional incorporation step can follow the identifying step. A polymerase different from the ones used in the binding and examination steps can be used to incorporate a nucleotide, such as a reversible terminator nucleotide, preliminary to identification of the next cognate nucleotide.

公开(公告)号：US20180127811A1

公开(公告)日：2018-05-10

申请号：US15707157

申请日：2017-09-18

申请人： Life Technologies Corporation

发明人： Scott C. Benson ,  Cinna Monighetti ,  Sandy M. Koepf

IPC分类号： C12Q1/6818 ,  C12Q1/6844 ,  C09B15/00 ,  C12Q1/6851

CPC分类号： C12Q1/6844 ,  C09B15/00 ,  C12Q1/6818 ,  C12Q1/6851 ,  C12Q2527/125 ,  C12Q2545/101 ,  C12Q2561/113 ,  C12Q2563/107

摘要： According to the present teachings, methods and compositions are provided that utilize at least one reference dye of formula (I): In some embodiments, a method comprises measuring a detection signal of a reporter dye and at least one reference dye of formula (I). In some embodiments, a composition comprises a reference dye of formula (1), a buffer, a selection of nucleotides and a protein.

公开(公告)号：US20180073064A1

公开(公告)日：2018-03-15

申请号：US15705821

申请日：2017-09-15

申请人： Roche Molecular Systems, Inc.

发明人： Igor Kozlov ,  Amar Gupta ,  Randall Saiki ,  Alison Tsan

IPC分类号： C12Q1/68

CPC分类号： C12Q1/686 ,  C12Q1/6804 ,  C12Q1/6811 ,  C12Q1/6818 ,  C12Q1/6823 ,  C12Q1/6837 ,  C12Q1/6883 ,  C12Q2531/113 ,  C12Q2537/143 ,  C12Q2561/113 ,  C12Q2600/16 ,  C12Q2525/161 ,  C12Q2527/107 ,  C12Q2565/101

摘要： The present invention describes methods for performing higher multiplexed real-time PCR for detection and quantitation of target nucleic acids using tagged hydrolysis probes.

公开(公告)号：US20180044714A1

公开(公告)日：2018-02-15

申请号：US15553091

申请日：2016-02-23

申请人： KYOTO UNIVERSITY

发明人： Mitinori SAITOU ,  Tomonori NAKAMURA ,  Yukihiro YABUTA

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6806 ,  C12Q1/68 ,  C12Q1/6851 ,  C12Q1/686 ,  C12Q1/6874 ,  C12Q2525/179 ,  C12Q2561/113

摘要： The invention provides a method of preparing a nucleic acid population suitable for RNA sequencing. The method involves amplifying a double-stranded DNA and a poly T sequence by using the DNA constituted of any additional nucleic acid sequence X, poly T sequence, mRNA sequence isolated from a biological sample, poly A sequence and any additional nucleic acid sequence Y in this order as a template, a first primer containing any additional nucleic acid sequence X having amine added to the 5′-terminal (and a poly T sequence), and a second primer containing any additional nucleic acid sequence Y (and a poly T sequence), followed by fractionalizing the DNA, phosphorylating the DNA, preparing cDNA by using the DNA as a template and a third primer, adding adenine (A) to the cDNA, linking a DNA, and amplifying the DNA by using the DNA as a template, a fourth primer, and a fifth primer.

公开(公告)号：US20170335408A1

公开(公告)日：2017-11-23

申请号：US15459666

申请日：2017-03-15

申请人： Abbott Laboratories

发明人： Frank Pawlowski ,  Joseph P. Skinner ,  Jack Kessler ,  Sonal Sadaria Nana

IPC分类号： C12Q1/68 ,  B01L9/00 ,  G01N30/88 ,  G01N35/00 ,  G01N33/569

CPC分类号： C12Q1/6888 ,  B01L9/523 ,  C12Q1/6806 ,  G01N30/88 ,  G01N33/48 ,  G01N33/56983 ,  G01N35/00 ,  G01N35/00584 ,  G01N2035/00326 ,  C12Q2527/113 ,  C12Q2531/113 ,  C12Q2545/114 ,  C12Q2561/113

摘要： The instant disclosure provides methods of multi-assay processing and multi-assay analysis. Such multi-assay processing and analysis pertain to automated detection of target nucleic acids, e.g., as performed in the clinical setting for diagnostic purposes. Also provided are common assay timing protocols derived from a variety of individual nucleic acid amplification and analysis protocols and modified to prevent resource contention. The instant disclosure also provides systems and devices for practicing the methods as described herein.

公开(公告)号：US09790544B2

公开(公告)日：2017-10-17

申请号：US15066248

申请日：2016-03-10

申请人： LIFE TECHNOLOGIES CORPORATION

发明人： Scott C. Benson ,  Cinna Monighetti ,  Sandy M. Koepf

IPC分类号： C12Q1/68 ,  C12P19/34 ,  C09B15/00

CPC分类号： C12Q1/6818 ,  C09B15/00 ,  C12Q1/6844 ,  C12Q1/6851 ,  C12Q2527/125 ,  C12Q2545/101 ,  C12Q2561/113 ,  C12Q2563/107

摘要： According to the present teachings, methods and compositions are provided that utilize at least one reference dye of formula (I): In some embodiments, a method comprises measuring a detection signal of a reporter dye and at least one reference dye of formula (I). In some embodiments, a composition comprises a reference dye of formula (1), a buffer, a selection of nucleotides and a protein.

公开(公告)号：US09777321B2

公开(公告)日：2017-10-03

申请号：US15260773

申请日：2016-09-09

申请人： Industrial Technology Research Institute

发明人： Chung-Fan Chiou ,  Ying-Chih Pu ,  Chao-Chi Pan ,  Chih-Tsung Shih ,  Ming-Chia Li ,  Chein-Shiu Kuo ,  Hung-Chi Chien ,  Chang-Sheng Chu

IPC分类号： G01N21/00 ,  C12Q1/68 ,  G01N21/64 ,  G01N21/77

CPC分类号： C12Q1/6869 ,  G01N21/6428 ,  G01N21/6452 ,  G01N21/6454 ,  G01N21/648 ,  G01N21/7703 ,  G01N2021/6439 ,  G01N2201/08 ,  G01N2201/12 ,  C12Q2563/155 ,  C12Q2561/113

摘要： Embodiments encompass a single-molecule detection system and methods of using the detection system to detect an object. Further, embodiments encompass a detection system comprising a movable light coupler, a waveguide, and a light detector. Embodiments further encompass methods of single-molecule detection, including methods of single-molecule nucleic acid sequencing.