公开(公告)号：US07385709B2

公开(公告)日：2008-06-10

申请号：US10501637

申请日：2003-01-15

申请人： Karsten Plamann ,  Stéphane Bourquin ,  Mathieu Ducros ,  Jelena Mitic ,  Francois Vuille ,  Theo Lasser ,  Tiemo Anhut

发明人： Karsten Plamann ,  Stéphane Bourquin ,  Mathieu Ducros ,  Jelena Mitic ,  Francois Vuille ,  Theo Lasser ,  Tiemo Anhut

IPC分类号： G01B11/24

CPC分类号： G02B21/06 ,  G02B21/002 ,  G02B21/367 ,  G02B27/58

摘要： A method and a microscopy imaging apparatus for generating an optically sectioned image of a specimen are provided. The method comprises the steps of: illuminating the specimen with a modulating, spatially periodic illumination pattern; imaging said specimen on a conjugate image plane; acquiring a plurality of signals at respective positions on said image plane, each signal corresponding to the incident light intensity at that position and having an oscillatory component caused by the modulation of the illumination pattern; and measuring a characteristic of the oscillatory component of each of the signals, whereby the measured characteristics when combined in their relative positions generate an optically sectioned image of the specimen.

摘要翻译： 提供了一种用于产生样本的光学截面图像的方法和显微镜成像设备。 该方法包括以下步骤：用调制的空间周期照明图案照射样本; 在共轭图像平面上成像所述样本; 在所述图像平面上的各个位置处获取多个信号，每个信号对应于该位置处的入射光强度并且具有由照明图案的调制引起的振荡分量; 并且测量每个信号的振荡分量的特性，由此当在其相对位置中组合时的测量特性产生样本的光学截面图像。

公开(公告)号：US20050117172A1

公开(公告)日：2005-06-02

申请号：US10501637

申请日：2003-01-15

申请人： Karsten Plamann ,  Stephane Bourquin ,  Mathieu Ducros ,  Jelena Mitic ,  Francois Vuille ,  Theo Lasser ,  Tiemo Anhut

发明人： Karsten Plamann ,  Stephane Bourquin ,  Mathieu Ducros ,  Jelena Mitic ,  Francois Vuille ,  Theo Lasser ,  Tiemo Anhut

IPC分类号： G02B26/00 ,  F03D7/02 ,  G02B21/00 ,  G02B21/06 ,  G02B21/36 ,  G01B11/24

CPC分类号： G02B21/06 ,  G02B21/002 ,  G02B21/367 ,  G02B27/58

摘要： A method and a microscopy imaging apparatus for generating an optically sectioned image of a specimen are provided. The method comprises the steps of: illuminating the specimen with a modulating, spatially periodic illumination pattern; imaging said specimen on a conjugate image plane; acquiring a plurality of signals at respective positions on said image plane, each signal corresponding to the incident light intensity at that position and having an oscillatory component caused by the modulation of the illumination pattern; and measuring a characteristic of the oscillatory component of each of the signals, whereby the measured characteristics when combined in their relative positions generate an optically sectioned image of the specimen.

摘要翻译： 提供了一种用于产生样本的光学截面图像的方法和显微镜成像设备。 该方法包括以下步骤：用调制的空间周期照明图案照射样本; 在共轭图像平面上成像所述样本; 在所述图像平面上的各个位置处获取多个信号，每个信号对应于该位置处的入射光强度并且具有由照明图案的调制引起的振荡分量; 并且测量每个信号的振荡分量的特性，由此当在其相对位置中组合时的测量特性产生样本的光学截面图像。