公开(公告)号：US08232447B2

公开(公告)日：2012-07-31

申请号：US12525410

申请日：2007-11-30

申请人： Katsumi Aragane ,  Katsuya Ohbuchi ,  Yoshitaka Tamai ,  Naomoto Harada ,  Naomi Murai ,  Yoshiki Ito ,  Jun Kusunoki ,  Yukina Tokushima ,  Takaharu Maruyama

发明人： Katsumi Aragane ,  Katsuya Ohbuchi ,  Yoshitaka Tamai ,  Naomoto Harada ,  Naomi Murai ,  Yoshiki Ito ,  Jun Kusunoki ,  Yukina Tokushima ,  Takaharu Maruyama

IPC分类号： A01K67/027 ,  A01K67/00 ,  C07H21/02 ,  C07H21/04

CPC分类号： G01N33/5088 ,  A01K67/0276 ,  A01K2217/075 ,  A01K2227/105 ,  A01K2267/0362 ,  C12N9/1051 ,  C12N15/8509 ,  C12N2800/30 ,  G01N2333/91051 ,  G01N2500/00 ,  G01N2800/044

摘要： It is an object to provide a gene-modified non-human animal having inactivated MGAT2 gene and a gene-modified non-human animal cell, which are useful for the search of the function of MGAT2 in vivo. It is another object to provide a method for screening of a compound capable of inhibiting the activity of MGAT2 and a compound capable of inhibiting the activity of MGAT2. It is further another object to provide a method for detecting a disease induced by abnormal lipid metabolism based on the expression level or activity of MGAT2. A method for screening of a compound by using a gene-modified non-human mammal having the artificially inhibited expression of MGAT2 gene and a cell thereof enable to prevent or treat a disease induced by abnormal lipid metabolism. Also a screening of a compound capable of inhibiting or enhancing the function of MGAT2 enables to prevent or treat a disease induced by abnormal lipid metabolism.

摘要翻译： 本发明的目的是提供具有失活的MGAT2基因和基因修饰的非人动物细胞的基因修饰的非人动物，其可用于在体内搜索MGAT2的功能。 另一个目的是提供一种筛选能够抑制MGAT2活性的化合物和能抑制MGAT2活性的化合物的方法。 另一个目的是提供一种基于MGAT2的表达水平或活性来检测由脂质代谢异常引起的疾病的方法。 通过使用人体抑制MGAT2基因表达的基因修饰的非人哺乳动物及其细胞筛选化合物的方法能够预防或治疗由脂质代谢异常引起的疾病。 另外，能够抑制或增强MGAT2功能的化合物的筛选能够预防或治疗由脂质代谢异常引起的疾病。

公开(公告)号：US20100074888A1

公开(公告)日：2010-03-25

申请号：US12525410

申请日：2007-11-30

申请人： Katsumi Aragane ,  Ktsuya Ohbuchi ,  Yoshitaka Tamai ,  Naomoto Harada ,  Naomi Murai ,  Yoshiki Ito ,  Jun Kusunoki ,  Yukina Tokushima ,  Takaharu Maruyama

发明人： Katsumi Aragane ,  Ktsuya Ohbuchi ,  Yoshitaka Tamai ,  Naomoto Harada ,  Naomi Murai ,  Yoshiki Ito ,  Jun Kusunoki ,  Yukina Tokushima ,  Takaharu Maruyama

IPC分类号： A61K39/395 ,  A01K67/027 ,  G01N33/50 ,  C12Q1/02 ,  A61K38/16 ,  A61K31/7052 ,  A61K36/00 ,  A61K35/00 ,  A61P3/06

CPC分类号： G01N33/5088 ,  A01K67/0276 ,  A01K2217/075 ,  A01K2227/105 ,  A01K2267/0362 ,  C12N9/1051 ,  C12N15/8509 ,  C12N2800/30 ,  G01N2333/91051 ,  G01N2500/00 ,  G01N2800/044

摘要： It is an object to provide a gene-modified non-human animal having inactivated MGAT2 gene and a gene-modified non-human animal cell, which are useful for the search of the function of MGAT2 in vivo. It is another object to provide a method for screening of a compound capable of inhibiting the activity of MGAT2 and a compound capable of inhibiting the activity of MGAT2. It is further another object to provide a method for detecting a disease induced by abnormal lipid metabolism based on the expression level or activity of MGAT2. A method for screening of a compound by using a gene-modified non-human mammal having the artificially inhibited expression of MGAT2 gene and a cell thereof enable to prevent or treat a disease induced by abnormal lipid metabolism. Also a screening of a compound capable of inhibiting or enhancing the function of MGAT2 enables to prevent or treat a disease induced by abnormal lipid metabolism.

摘要翻译： 本发明的目的是提供具有失活的MGAT2基因和基因修饰的非人动物细胞的基因修饰的非人动物，其可用于在体内搜索MGAT2的功能。 另一个目的是提供一种筛选能够抑制MGAT2活性的化合物和能抑制MGAT2活性的化合物的方法。 另一个目的是提供一种基于MGAT2的表达水平或活性来检测由脂质代谢异常引起的疾病的方法。 通过使用人体抑制MGAT2基因表达的基因修饰的非人哺乳动物及其细胞筛选化合物的方法能够预防或治疗由脂质代谢异常引起的疾病。 另外，能够抑制或增强MGAT2功能的化合物的筛选能够预防或治疗由脂质代谢异常引起的疾病。