公开(公告)号：US5071760A

公开(公告)日：1991-12-10

申请号：US656122

申请日：1991-02-14

申请人： Katsuto Watanabe ,  Yoshihiko Nakamura ,  Takashi Noto ,  Masaichi Yamamura ,  Hitoshi Nakashima ,  Kazunori Ichinohe ,  Yukitaka Mino ,  Kazuhiro Nishijima

发明人： Katsuto Watanabe ,  Yoshihiko Nakamura ,  Takashi Noto ,  Masaichi Yamamura ,  Hitoshi Nakashima ,  Kazunori Ichinohe ,  Yukitaka Mino ,  Kazuhiro Nishijima

IPC分类号： B01F9/00 ,  B01F11/00 ,  C12M1/00 ,  C12M3/00

CPC分类号： B01F9/0001 ,  B01F11/0062 ,  C12M23/14 ,  C12M25/02 ,  C12M27/10 ,  C12M27/16 ,  C12M29/04 ,  Y10S604/903

摘要： The instant invention relates to a method for culturing and floating animal cells in a double-bag container, where an outer bag containing culture media and a volume of air houses an inner bag of a semipermeable film containing cells suspended in a culture media. The semipermeable film has pores of sufficient size so as to prohibit cells within the inner bag from passing therethrough but allowing culture liquid and air to pass through it. To optimize the exchange of nutrients between the interiors of the two bags, a protective mesh surrounds the inner bag and the double-bag container is fastened to an agitator which provides either a rotating or shaking motion.

公开(公告)号：US5057429A

公开(公告)日：1991-10-15

申请号：US349701

申请日：1989-05-10

申请人： Katsuto Watanabe ,  Yoshihiko Nakamura ,  Takashi Noto ,  Masaichi Yamamura ,  Hitoshi Nakashima ,  Kazunori Ichinohe ,  Yukitaka Mino ,  Kazuhiro Nishijima

发明人： Katsuto Watanabe ,  Yoshihiko Nakamura ,  Takashi Noto ,  Masaichi Yamamura ,  Hitoshi Nakashima ,  Kazunori Ichinohe ,  Yukitaka Mino ,  Kazuhiro Nishijima

IPC分类号： B01F9/00 ,  B01F11/00 ,  C12M1/00 ,  C12M3/00 ,  C12M3/02

CPC分类号： B01F9/0001 ,  B01F11/0062 ,  C12M23/14 ,  C12M25/02 ,  C12M27/10 ,  C12M27/16 ,  C12M29/04 ,  Y10S604/903

摘要： The instant invention relates to an apparatus for floating animal cells in a double-bag container, where an outer bag containing culture media and a volume of air houses an inner bag of a semipermeable film containing cells suspended in a culture media. The semipermeable film has pores of sufficient size so as to prohibit cells within the inner bag from passing therethrough but allowing culture liquid and air to pass through it. To optimize the exchange of nutrients between the interiors of the two bags, a protective mesh surrounds the inner bag and the double-bag container is fastened to an agitator which provides either a rotating or shaking motion.

摘要翻译： 本发明涉及一种用于将动物细胞悬浮在双袋容器中的装置，其中包含培养基和一定体积的空气的外袋容纳含有悬浮在培养基中的细胞的半透膜的内袋。 半透膜具有足够尺寸的孔，以防止内袋中的细胞通过，但允许培养液和空气通过。 为了优化两个袋子的内部之间的营养物质的交换，保护网围绕内袋，并且双袋容器被紧固到提供旋转或摇动运动的搅拌器。