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    METHOD FOR SELECTIVELY RECOVERING C-TERMINAL PEPTIDE OF PROTEIN AND METHOD FOR DETERMINING AMINO ACID SEQUENCE OF C-TERMINAL PEPTIDE OF PROTEIN USING THE SAME
    1.
    发明申请
    METHOD FOR SELECTIVELY RECOVERING C-TERMINAL PEPTIDE OF PROTEIN AND METHOD FOR DETERMINING AMINO ACID SEQUENCE OF C-TERMINAL PEPTIDE OF PROTEIN USING THE SAME 审中-公开
    用于选择性恢复蛋白质C端蛋白的方法和使用该蛋白质确定蛋白质C-末端肽的氨基酸序列的方法

    公开(公告)号：US20090142851A1

    公开(公告)日：2009-06-04

    申请号：US12325115

    申请日：2008-11-28

    申请人： Keisuke Shima ,  Minoru Yamaguchi ,  Hiroki Kuyama ,  Eiji Ando ,  Osamu Nishimura ,  Susumu Tsunasawa ,  Kazuhiro Sonomura

    发明人： Keisuke Shima ,  Minoru Yamaguchi ,  Hiroki Kuyama ,  Eiji Ando ,  Osamu Nishimura ,  Susumu Tsunasawa ,  Kazuhiro Sonomura

    IPC分类号： G01N33/68 ,  C07K1/14 ,  C12P21/06

    CPC分类号： C07K1/1072 ,  C07K1/128 ,  G01N33/6821

    摘要： The present invention provides a method for specifically recovering a C-terminal peptide fragment, and a method for easily determining the sequence of a C-terminal peptide fragment, which is difficult to be determined by a conventional method, with the use of a mass spectrometer, in particular a method capable of de novo sequencing of a C-terminal peptide fragment. A method for selectively recovering a C-terminal peptide of a protein, comprising the steps of: in a cleavage product of a protein containing a C-terminal peptide fragment (A) having an α-amino group but not having an ε-amino group and the other peptide fragments (B) having an α-amino group and an ε-amino group, selectively modifying the α-amino groups to obtain a C-terminal peptide fragment modified (A′) and the other peptide fragments modified (B′); and separating the C-terminal peptide fragment modified (A′) from the modified cleavage product by allowing a carrier to hold the other peptide fragments modified (B′) via the ε-amino group. A method for determining the amino acid sequence of a C-terminal peptide of a protein, comprising the steps of: selectively recovering a C-terminal peptide of a protein by the above method; and determining the amino acid sequence by subjecting a recovered C-terminal peptide fragment to mass spectrometry measurement.

    摘要翻译： 本发明提供了特异性回收C末端肽片段的方法，以及通过使用质谱仪容易地测定难以用常规方法测定的C末端肽片段的序列的方法 ，特别是能够重新测序C-末端肽片段的方法。 一种选择性回收蛋白质C末端肽的方法，包括以下步骤：在含有具有α-氨基但不具有ε-氨基的C-末端肽片段（A）的蛋白质的切割产物中 和具有α-氨基和ε-氨基的其它肽片段（B），选择性地修饰α-氨基以获得修饰的C末端肽片段（A'）和修饰的其它肽片段（B' ）; 以及通过允许载体通过ε-氨基保持修饰的其它肽片段（B'），从修饰的切割产物中分离修饰的C末端肽片段（A'）。 一种确定蛋白质C-末端肽的氨基酸序列的方法，包括以下步骤：通过上述方法选择性地回收蛋白质的C-末端肽; 并通过对回收的C-末端肽片段进行质谱测量来测定氨基酸序列。

    Motor function estimating system, motor function estimating method and program
    4.
    发明授权
    Motor function estimating system, motor function estimating method and program 有权
    电机功能估算系统，电机功能估算方法及程序

    公开(公告)号：US08574173B2

    公开(公告)日：2013-11-05

    申请号：US12854357

    申请日：2010-08-11

    申请人： Saburo Sakoda ,  Toshio Tsuji ,  Keisuke Shima ,  Masaru Yokoe ,  Yuko Sano ,  Akihiko Kandori ,  Tsuyoshi Miyashita

    发明人： Saburo Sakoda ,  Toshio Tsuji ,  Keisuke Shima ,  Masaru Yokoe ,  Yuko Sano ,  Akihiko Kandori ,  Tsuyoshi Miyashita

    IPC分类号： A61B5/103 ,  A61B5/117 ,  A61B5/00 ,  G01L5/16

    CPC分类号： A61B5/1101 ,  A61B5/4082 ,  A61B5/6826 ,  A61B5/6838 ,  A61B5/7239

    摘要： The motor function estimating system 1000 has a memory unit 1260, a data processing unit 1220 (analyzing section), and a display unit 1400. The memory unit 1260 memorizes waveform data about the time change of fingers tapping motion that is obtained by a motion sensor attached to a subject person who does the fingers tapping motion that is repetition of the opening and closing motion of two fingers of one hand. The data processing unit 1220 analyzes the waveform data in the memory unit 1260. The display unit 1400 displays an analysis result. The data processing unit 1220 creates motion waveform based on waveform data, and plural characteristics based on the motion waveform. The data processing unit 1220 creates a motion disorder synthesis value that represents degree of motion disorder of the subject person by revising each of the plural characteristics based on characteristics of every age and synthesizing them.

    摘要翻译： 电动机功能估计系统1000具有存储单元1260，数据处理单元1220（分析单元）和显示单元1400.存储单元1260存储关于由运动传感器获得的手指敲击运动的时间变化的波形数据 附着在手指敲击运动的对象人身上，这是一只手的两个手指的开启和关闭运动的重复。 数据处理单元1220分析存储单元1260中的波形数据。显示单元1400显示分析结果。 数据处理单元1220基于波形数据创建运动波形，并且基于运动波形产生多个特性。 数据处理单元1220通过基于每个年龄的特征修改每个特征并合成来创建表示被摄体人的运动障碍的运动障碍合成值。