公开(公告)号：US20090233322A1

公开(公告)日：2009-09-17

申请号：US12469403

申请日：2009-05-20

申请人： Kenji NAGAKAWA ,  Tomomichi TSUJIMOTO ,  Susumu NISHINO ,  Masaaki TERAMOTO ,  Yoshiyuki KAWASE

发明人： Kenji NAGAKAWA ,  Tomomichi TSUJIMOTO ,  Susumu NISHINO ,  Masaaki TERAMOTO ,  Yoshiyuki KAWASE

IPC分类号： C12Q1/26 ,  C12N9/02

CPC分类号： G01N21/78 ,  C12Q1/26 ,  C12Q1/32

摘要： The present invention provides a colorimetric method that can perform a simple and reliable analysis in a short time. The method includes transferring an electron from an analyte to a coloring reagent that produces color by reduction via a mediator by using an oxidoreductase; and performing qualitative or quantitative analysis of the analyte by measuring color produced in the coloring reagent. The enzyme reaction of this method is a single stage reaction, and the color production reaction occurs via the mediator. Therefore, the measurement can be performed in a short time. Since this reaction requires neither hydrogen peroxide nor oxygen, the measured values are highly reliable.

摘要翻译： 本发明提供一种可以在短时间内进行简单可靠的分析的比色法。 该方法包括将电子从分析物转移到通过使用氧化还原酶通过介质还原产生颜色的着色剂; 并通过测量着色剂中产生的颜色来对分析物进行定性或定量分析。 该方法的酶反应是单阶段反应，并且通过介体发生颜色产生反应。 因此，可以在短时间内进行测量。 由于该反应既不需要过氧化氢也不需要氧气，因此测量值非常可靠。