公开(公告)号：US20090215860A1

公开(公告)日：2009-08-27

申请号：US11570822

申请日：2005-06-17

申请人： Kevin V. Morris ,  David J. Looney ,  Steven E. Jacobsen

发明人： Kevin V. Morris ,  David J. Looney ,  Steven E. Jacobsen

IPC分类号： A61K31/7105 ,  C12Q1/68 ,  C12Q1/70 ,  C12N5/00

CPC分类号： C12N15/111 ,  C12N15/113 ,  C12N15/1131 ,  C12N15/1132 ,  C12N15/1133 ,  C12N2310/111 ,  C12N2310/152 ,  C12N2310/351 ,  C12N2320/10 ,  C12N2320/30 ,  C12N2799/027

摘要： The invention is directed to compositions and methods for RNA-mediated gene regulation, e.g., transcription regulation, e.g., by transcriptional silencing of genes. In one aspect, the invention provides methods using siRNAs directed at a transcription regulator, e.g., a promoter or enhance sequence, of a gene target molecule. In one aspect, this results in in vivo DNA methylation and/or modification of associated chromatin (e.g., histone proteins) accompanied by and/or partial or complete transcription gene silencing in a cell, such as a mammalian cell, e.g., a human cell.

摘要翻译： 本发明涉及用于RNA介导的基因调节的组合物和方法，例如转录调节，例如通过基因的转录沉默。 在一个方面，本发明提供使用针对基因靶分子的转录调节子例如启动子或增强序列的siRNA的方法。 一方面，这导致在细胞如哺乳动物细胞（例如人细胞）中伴随着和/或部分或完全转录基因沉默的相关染色质（例如，组蛋白）的体内DNA甲基化和/或修饰 。

公开(公告)号：US08513401B2

公开(公告)日：2013-08-20

申请号：US12772652

申请日：2010-05-03

申请人： John J. Rossi ,  Daniela Castanotto ,  Gerd Pfeifer ,  Stella Tommasi ,  Kevin V. Morris ,  Daniel H. Kim

发明人： John J. Rossi ,  Daniela Castanotto ,  Gerd Pfeifer ,  Stella Tommasi ,  Kevin V. Morris ,  Daniel H. Kim

IPC分类号： C07H21/04 ,  C07H21/02

CPC分类号： C12N15/111 ,  C12N15/1135 ,  C12N15/63 ,  C12N2310/111 ,  C12N2310/14 ,  C12N2310/53

摘要： The present invention relates to transcriptional gene silencing (TGS) in mammalian, including human, cells that is mediated by small interfering RNA (siRNA) molecules. The present invention also relates to a double stranded nucleic acid that directs methylation of histones associated with target genes that produce low copy promoter-specific RNA. It has been found that siRNAs can be used to direct methylation of histones in mammalian, including human, cells.

摘要翻译： 本发明涉及哺乳动物（包括人）由小干扰RNA（siRNA）分子介导的细胞的转录基因沉默（TGS）。 本发明还涉及引导与产生低拷贝启动子特异性RNA的靶基因相关的组蛋白甲基化的双链核酸。 已经发现siRNA可以用于引导哺乳动物（包括人）细胞中组蛋白的甲基化。