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1.发明授权公开(公告)号：US08975028B2
公开(公告)日：2015-03-10
申请号：US14219931
申请日：2014-03-19
Applicant: Keygene N.V.
Inventor： Michael Josephus Theresia Van Eijk , Taco Peter Jesse
CPC classification number: C12Q1/6874 , C12Q1/6869 , C12Q2535/138
Abstract: The present invention relates to a high throughput method for the identification and detection of molecular markers wherein restriction fragments are generated and suitable adaptors comprising (sample-specific) identifiers are ligated. The adapter-ligated restriction fragments may be selectively amplified with adaptor compatible primers carrying selective nucleotides at their 3′ end. The amplified adapter-ligated restriction fragments are, at least partly, sequenced using high throughput sequencing methods and the sequence parts of the restriction fragments together with the sample-specific identifiers serve as molecular markers.
Abstract translation: 本发明涉及用于识别和检测其中产生限制性片段的分子标记物的高通量方法，并且包括（样品特异性）标识符的合适的衔接子被连接。 衔接子连接的限制性片段可以用在3'末端携带选择性核苷酸的衔接子兼容引物进行选择性扩增。 使用高通量测序方法至少部分地测序扩增的接头连接的限制性片段，并且限制性片段的序列部分与样品特异性标识符一起用作分子标记。
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Patent Agency Ranking
2.发明授权公开(公告)号：US08685650B2
公开(公告)日：2014-04-01
申请号：US13783601
申请日：2013-03-04
Applicant: Keygene N.V.
Inventor： Michael Josephus Theresia Van Eijk , Taco Peter Jesse
CPC classification number: C12Q1/6874 , C12Q1/6869 , C12Q2535/138
Abstract: The present invention relates to a high throughput method for the identification and detection of molecular markers wherein restriction fragments are generated and suitable adaptors comprising (sample-specific) identifiers are ligated. The adapter-ligated restriction fragments may be selectively amplified with adaptor compatible primers carrying selective nucleotides at their 3′ end. The amplified adapter-ligated restriction fragments are, at least partly, sequenced using high throughput sequencing methods and the sequence parts of the restriction fragments together with the sample-specific identifiers serve as molecular markers.
Abstract translation: 本发明涉及用于识别和检测其中产生限制性片段的分子标记物的高通量方法，并且包括（样品特异性）标识符的合适的衔接子被连接。 衔接子连接的限制性片段可以用在3'末端携带选择性核苷酸的衔接子兼容引物进行选择性扩增。 使用高通量测序方法至少部分地测序扩增的接头连接的限制性片段，并且限制性片段的序列部分与样品特异性标识符一起用作分子标记。
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公开(公告)号：US10316364B2
公开(公告)日：2019-06-11
申请号：US15707650
申请日：2017-09-18
Applicant: Keygene N.V.
IPC: C12Q1/68 , C12Q1/6874 , C12Q1/6869
Abstract: The present invention relates to a method for identifying the source of an amplicon, comprising: providing a plurality of pools of amplicons from different sources, wherein the amplicons from different sources are present in more than one pool, and wherein the amplicons in each pool are tagged with a unique pool-specific identifier; sequencing at least part of the amplicons that comprise the pool-specific identifiers; and assigning one or more of the amplicons to corresponding pools and/or sources using the pool-specific identifiers.
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4.发明申请公开(公告)号：US20130184166A1
公开(公告)日：2013-07-18
申请号：US13783601
申请日：2013-03-04
Applicant: Keygene N.V.
Inventor： Michael Josephus Theresia VAN EIJK , Taco Peter Jesse
IPC: C12Q1/68
CPC classification number: C12Q1/6874 , C12Q1/6869 , C12Q2535/138
Abstract: The present invention relates to a high throughput method for the identification and detection of molecular markers wherein restriction fragments are generated and suitable adaptors comprising (sample-specific) identifiers are ligated. The adapter-ligated restriction fragments may be selectively amplified with adaptor compatible primers carrying selective nucleotides at their 3′ end. The amplified adapter-ligated restriction fragments are, at least partly, sequenced using high throughput sequencing methods and the sequence parts of the restriction fragments together with the sample-specific identifiers serve as molecular markers.
Abstract translation: 本发明涉及用于识别和检测其中产生限制性片段的分子标记物的高通量方法，并且包括（样品特异性）标识符的合适的衔接子被连接。 衔接子连接的限制性片段可以用在3'末端携带选择性核苷酸的衔接子兼容引物进行选择性扩增。 使用高通量测序方法至少部分地测序扩增的接头连接的限制性片段，并且限制性片段的序列部分与样品特异性标识符一起用作分子标记。
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公开(公告)号：US09284606B2
公开(公告)日：2016-03-15
申请号：US14613849
申请日：2015-02-04
Applicant: Keygene N.V.
Inventor： Michael Josephus Theresia Van Eijk , Taco Peter Jesse
CPC classification number: C12Q1/6874 , C12Q1/6869 , C12Q2535/138
Abstract: The present invention relates to a high throughput method for the identification and detection of molecular markers wherein restriction fragments are generated and suitable adaptors comprising (sample-specific) identifiers are ligated. The adapter-ligated restriction fragments may be selectively amplified with adaptor compatible primers carrying selective nucleotides at their 3′ end. The amplified adapter-ligated restriction fragments are, at least partly, sequenced using high throughput sequencing methods and the sequence parts of the restriction fragments together with the sample-specific identifiers serve as molecular markers.
Abstract translation: 本发明涉及用于识别和检测其中产生限制性片段的分子标记物的高通量方法，并且包括（样品特异性）标识符的合适的衔接子被连接。 衔接子连接的限制性片段可以用在3'末端携带选择性核苷酸的衔接子兼容引物进行选择性扩增。 使用高通量测序方法至少部分地测序扩增的接头连接的限制性片段，并且限制性片段的序列部分与样品特异性标识符一起用作分子标记。
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6.发明申请公开(公告)号：US20140206551A1
公开(公告)日：2014-07-24
申请号：US14219931
申请日：2014-03-19
Applicant: Keygene N.V.
Inventor： Michael Josephus Theresia VAN EIJK , Taco Peter Jesse
IPC: C12Q1/68
CPC classification number: C12Q1/6874 , C12Q1/6869 , C12Q2535/138
Abstract: The present invention relates to a high throughput method for the identification and detection of molecular markers wherein restriction fragments are generated and suitable adaptors comprising (sample-specific) identifiers are ligated. The adapter-ligated restriction fragments may be selectively amplified with adaptor compatible primers carrying selective nucleotides at their 3′ end. The amplified adapter-ligated restriction fragments are, at least partly, sequenced using high throughput sequencing methods and the sequence parts of the restriction fragments together with the sample-specific identifiers serve as molecular markers.
Abstract translation: 本发明涉及用于识别和检测其中产生限制性片段的分子标记物的高通量方法，并且包括（样品特异性）标识符的合适的衔接子被连接。 衔接子连接的限制性片段可以用在3'末端携带选择性核苷酸的衔接子兼容引物进行选择性扩增。 使用高通量测序方法至少部分地测序扩增的接头连接的限制性片段，并且限制性片段的序列部分与样品特异性标识符一起用作分子标记。
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