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    Rapid prion-detection assay
    1.
    发明授权
    Rapid prion-detection assay 失效
    快速朊病毒检测法

    公开(公告)号：US07045297B2

    公开(公告)日：2006-05-16

    申请号：US09993391

    申请日：2001-11-14

    申请人： Kiamars Hajizadeh ,  Zakir S. Murtaza

    发明人： Kiamars Hajizadeh ,  Zakir S. Murtaza

    IPC分类号： G01N33/53

    CPC分类号： G01N33/6896 ,  G01N2800/2828 ,  Y10S435/962 ,  Y10S435/97

    摘要： Assays are provided for rapid detection, with high specificity of the pathogenic form of prion protein responsible for neurodegenerative diseases affecting humans and animals, such as transmissible spongiform encephalopathy in bovine, sheep, and cats. Also provided are assays for testing animal feedstock, such as animal feed, for the presence or concentration of pathogenic prion protein. Results are available in from about 0.5 to about 20 minutes and preferably within from about 5 to about 10 minutes. The assays employ proteinase-K to remove normal prion protein from a biological sample, so that the sample may be analyzed by immunochromatography to determine the presence and concentration of pathogenic prion protein. Because the proteinase-K is immobilized on a solid support for in situ removal of interfering components, the present invention obviates the need for subsequent extraction of the desired analyte. All aspects of the present invention are suitable for quantifying the minimal detectable amount of pathogenic prion protein in a biological sample. Moreover, the simplicity of sample preparation makes the present invention suitable for use in the field.

    摘要翻译： 提供了用于快速检测的测定法，其具有导致影响人和动物的神经变性疾病如朊，羊和猫的传染性海绵状脑病的朊病毒蛋白的致病形式的高特异性。 还提供了用于测试动物原料（例如动物饲料）用于病原性朊​​病毒蛋白的存在或浓缩的测定。 结果可用约0.5至约20分钟，优选约5至约10分钟。 测定采用蛋白酶K从生物样品中除去正常的朊病毒蛋白，从而可以通过免疫色谱法分析样品，以确定致病性朊病毒蛋白的存在和浓度。 因为蛋白酶-K被固定在固体支持物上用于原位去除干扰组分，所以本发明消除了随后提取所需分析物的需要。 本发明的所有方面都适用于量化生物样品中最小可检测量的致病性朊病毒蛋白。 此外，样品制备的简单性使得本发明适用于本领域。