公开(公告)号：US20080108837A1

公开(公告)日：2008-05-08

申请号：US10579162

申请日：2004-11-09

申请人： Koji Nakanishi ,  Stanislav Jaracz ,  Shahid Malik ,  Hideki Ishii ,  Sergei V. Dzyuba

发明人： Koji Nakanishi ,  Stanislav Jaracz ,  Shahid Malik ,  Hideki Ishii ,  Sergei V. Dzyuba

IPC分类号： C07D407/14

CPC分类号： C07D493/22

摘要： The subject invention provides a method for separating a terpene trilactone from Ginkgo biloba plant material or from an extract of Ginkgo biloba comprising a mixture of terpene trilactones, the process comprising the steps of:a) subjecting the Ginkgo biloba plant material or the extract to column chromatography with an appropriate solvent system to produce at least a first fraction containing the terpene trilactone bilobalide, a second fraction eluted after the first fraction containing the terpene trilactones GA and GB, and a third fraction eluted after the second fraction containing at least a preponderance of the terpene trilactones GC and GJ; andb) alkylating the terpene trilactone GB of the second fraction so as to produce a first mixture including terpene trilactone GA and alkylated terpene trilactone GB; or alkylating the terpene trilactone GC of the third fraction so as produce a second mixture including terpene trilactone GJ and alkylated terpene trilactone GC, so as to thereby isolate a terpene trilactone.

摘要翻译： 本发明提供了从银杏植物材料或银杏提取物中分离萜烯三内酯的方法，其中包括萜内三聚物的混合物，该方法包括以下步骤：a）将银杏植物材料或提取物经柱 用适当的溶剂体系进行色谱以产生至少一种含有萜烯三内酰亚叶内酰脲的第一级分，在含有萜烯三内酰胺GA和GB的第一级分之后洗脱的第二级分，以及第二级分洗脱的第三级分，其中至少含有 萜类三内酯GC和GJ; 和b）将第二级分的萜烯三内酯GB烷基化，以产生包含萜内酯GA和烷基化萜三内酯GB的第一混合物; 或烷基化第三级分的萜烯三内酰胺GC，从而产生包括萜内酯GJ和烷基化萜三内酰胺GC的第二混合物，从而分离萜烯三内酯。

公开(公告)号：US07763741B2

公开(公告)日：2010-07-27

申请号：US10579162

申请日：2004-11-09

申请人： Koji Nakanishi ,  Stanislav Jaracz ,  Shahid Malik ,  Hideki Ishii ,  Sergei V. Dzyuba

发明人： Koji Nakanishi ,  Stanislav Jaracz ,  Shahid Malik ,  Hideki Ishii ,  Sergei V. Dzyuba

IPC分类号： C07D407/00

CPC分类号： C07D493/22

摘要： The subject invention provides a method for separating a terpene trilactone from Ginkgo biloba plant material or from an extract of Ginkgo biloba comprising a mixture of terpene trilactones, the process comprising the steps of: a) subjecting the Ginkgo biloba plant material or the extract to column chromatography with an appropriate solvent system to produce at least a first fraction containing the terpene trilactone bilobalide, a second fraction eluted after the first fraction containing the terpene trilactones GA and GB, and a third fraction eluted after the second fraction containing at least a preponderance of the terpene trilactones GC and GJ; and b) alkylating the terpene trilactone GB of the second fraction so as to produce a first mixture including terpene trilactone GA and alkylated terpene trilactone GB; or alkylating the terpene trilactone GC of the third fraction so as produce a second mixture including terpene trilactone GJ and alkylated terpene trilactone GC, so as to thereby isolate a terpene trilactone.

摘要翻译： 本发明提供了从银杏植物材料或银杏提取物中分离萜烯三内酯的方法，其中包括萜内三聚物的混合物，该方法包括以下步骤：a）将银杏植物材料或提取物经柱 用适当的溶剂体系进行色谱以产生至少一种含有萜烯三内酰亚叶内酰脲的第一级分，在含有萜烯三内酰胺GA和GB的第一级分洗脱的第二级分，以及第二级分洗脱的第三级分，其中至少含有 萜类三内酯GC和GJ; 和b）将第二级分的萜烯三内酯GB烷基化，以产生包含萜内酯GA和烷基化萜三内酯GB的第一混合物; 或烷基化第三级分的萜烯三内酰胺GC，从而产生包括萜内酯GJ和烷基化萜三内酰胺GC的第二混合物，从而分离萜烯三内酯。