公开(公告)号：US10031139B2

公开(公告)日：2018-07-24

申请号：US14387691

申请日：2013-03-28

Applicant: Konica Minolta, Inc. ,  Tohoku University

Inventor： Kensaku Takanashi ,  Hideki Gouda ,  Keizou Takano ,  Hideki Hoshino ,  Yasushi Nakano ,  Kohsuke Gonda ,  Noriaki Ohuchi ,  Mika Watanabe

IPC: G01N33/58 ,  G01N33/533 ,  G01N33/543 ,  B82Y30/00 ,  G01N1/30

Abstract: The present invention provides a staining method in which the fluorescent staining properties in a fluorescently-immunostained specimen are not reduced even when an oil-based mounting medium is used. The present invention also provides a method of preventing deterioration of a fluorescent label caused by irradiation with excitation light and improving the light resistance in a fluorescently-immunostained specimen obtained by the staining method. The biological substance detection method according to the present invention is a biological substance detection method for specifically detecting a biological substance from a pathological specimen, which includes the steps of: immunostaining the specimen with a fluorescent label; immobilizing the thus stained specimen; and mounting the thus immobilized specimen using a mounting medium including an organic solvent not freely miscible with water. In the biological substance detection method, the above-described mounting medium further includes a discoloration inhibitor.

公开(公告)号：US20150079611A1

公开(公告)日：2015-03-19

申请号：US14387691

申请日：2013-03-28

Applicant: Konica Minolta, Inc. ,  Tohoku University

Inventor： Kensaku Takanashi ,  Hideki Gouda ,  Keizou Takano ,  Hideki Hoshino ,  Yasushi Nakano ,  Kohsuke Gonda ,  Noriaki Ohuchi ,  Mika Watanabe

IPC: G01N33/543 ,  G01N33/58

CPC classification number: G01N33/54393 ,  B82Y30/00 ,  G01N1/30 ,  G01N33/533 ,  G01N33/582 ,  G01N33/585

Abstract: The present invention provides a staining method in which the fluorescent staining properties in a fluorescently-immunostained specimen are not reduced even when an oil-based mounting medium is used. The present invention also provides a method of preventing deterioration of a fluorescent label caused by irradiation with excitation light and improving the light resistance in a fluorescently-immunostained specimen obtained by the staining method. The biological substance detection method according to the present invention is a biological substance detection method for specifically detecting a biological substance from a pathological specimen, which includes the steps of: immunostaining the specimen with a fluorescent label; immobilizing the thus stained specimen; and mounting the thus immobilized specimen using a mounting medium including an organic solvent not freely miscible with water. In the biological substance detection method, the above-described mounting medium further includes a discoloration inhibitor.

Abstract translation: 本发明提供了即使使用油性安装介质时，荧光免疫染色试样中的荧光染色性也不会降低的染色方法。 本发明还提供一种防止由激发光照射引起的荧光标记物的劣化并提高通过染色方法获得的荧光免疫染色试样中的耐光性的方法。 本发明的生物体检测方法是从病理标本中特异性检测生物体的生物物质检测方法，其特征在于，包括以下步骤：用荧光标记物免疫染色标本; 固定如此染色的样品; 并使用包括不能与水自由混溶的有机溶剂的安装介质来安装如此固定的样品。 在生物物质检测方法中，上述安装介质还包括变色抑制剂。

公开(公告)号：US11435348B2

公开(公告)日：2022-09-06

申请号：US16715180

申请日：2019-12-16

Applicant: Konica Minolta, Inc.

Inventor： Hideki Gouda ,  Hisatake Okada ,  Yasushi Nakano ,  Kohsuke Gonda ,  Noriaki Ohuchi ,  Mika Watanabe

IPC: G01N33/569 ,  G01N1/30

Abstract: It is an object of the present invention to provide a tissue staining method that makes it possible to observe both information on the morphology of a tissue and information on a biological substance such as an antigen molecule to be detected on a single section and in a single view field. The present invention provides a tissue staining method, including carrying out (A) a HE (hematoxylin-eosin) staining, and (B) a histochemical staining, serially on a single tissue section, wherein the histochemical staining is defined as a histochemical technique for detecting a biological substance to be detected in a tissue in a visible manner by use of a binding reaction between the biological substance to be detected and a probe biological substance capable of binding specifically to the biological substance to be detected.

公开(公告)号：US11275087B2

公开(公告)日：2022-03-15

申请号：US15806970

申请日：2017-11-08

Applicant: Konica Minolta, Inc.

Inventor： Hideki Gouda ,  Masaru Takahashi ,  Kensaku Takanashi ,  Hisatake Okada ,  Yuichi Ozaki ,  Yuka Yoshihara ,  Yasushi Nakano ,  Kohsuke Gonda ,  Noriaki Ohuchi ,  Mika Watanabe ,  Norikazu Masuda ,  Masakazu Toi ,  Hiroshi Tada ,  Minoru Miyashita

IPC: G01N33/574 ,  G01N33/52 ,  G01N33/53 ,  G06K9/00 ,  G01N33/58 ,  G01N1/30 ,  G01N33/50

Abstract: The present invention relates to a test support method which is a means for predicting whether or not pathological complete response (pCR) will be attained when a preoperative chemotherapy with an anticancer drug is performed, the method using a sample (breast cancer tissue section) collected from a breast cancer patient to be subjected to the preoperative chemotherapy. The test support method includes: [1] the step of acquiring a fluorescence image of a breast cancer tissue section, which fluorescence image shows bright spots of fluorescent nanoparticles labeling one or more kinds of breast cancer-related proteins; [2] the step of acquiring at least one index relating to the expression level(s) of the breast cancer-related protein(s) on the basis of the bright spots of the fluorescence image; and [3] the step of acquiring information for predicting pCR by performing an analysis using the above-described at least one index.

公开(公告)号：US10309900B1

公开(公告)日：2019-06-04

申请号：US16256224

申请日：2019-01-24

Applicant: Konica Minolta, Inc.

Inventor： Hideki Gouda ,  Kensaku Takanashi ,  Kohsuke Gonda ,  Noriaki Ohuchi ,  Mika Watanabe

IPC: H04N7/18 ,  G06K9/00 ,  G01N21/64 ,  G01N33/483 ,  G01N33/58 ,  G06T7/90 ,  G01N21/93 ,  G06T7/00 ,  G01N33/53 ,  G01N33/48 ,  G01N33/50

Abstract: A biological substance quantitation method includes the following. A fluorescent image is input, which represents an expression of a specific biological substance in a sample stained with a fluorescent substance by a florescent bright spot. A quantitative evaluation value of the florescent bright spot is calculated. A standard fluorescent image of a standard sample stained under a same condition as the sample and representing an expression of the biological substance in a standard sample, is input under a same condition as the fluorescent image. A quantitative evaluation value in the standard fluorescent image is calculated under a same condition as the fluorescent image. Based on a correlation between an expression amount of the biological substance in a standard sample measured in advance and the evaluation value in the standard fluorescent image, the evaluation value in the fluorescent image is converted to an expression amount of the biological substance in the sample.

公开(公告)号：US10458983B2

公开(公告)日：2019-10-29

申请号：US16034593

申请日：2018-07-13

Applicant: Konica Minolta, Inc.

Inventor： Kensaku Takanashi ,  Hideki Gouda ,  Keizou Takano ,  Hideki Hoshino ,  Yasushi Nakano ,  Kohsuke Gonda ,  Noriaki Ohuchi ,  Mika Watanabe

IPC: G01N33/543 ,  G01N1/30 ,  G01N33/533 ,  G01N33/58 ,  B82Y30/00

Abstract: The present invention provides a staining method in which the fluorescent staining properties in a fluorescently-immunostained specimen are not reduced even when an oil-based mounting medium is used. The present invention also provides a method of preventing deterioration of a fluorescent label caused by irradiation with excitation light and improving the light resistance in a fluorescently-immunostained specimen obtained by the staining method. The biological substance detection method according to the present invention is a biological substance detection method for specifically detecting a biological substance from a pathological specimen, which includes the steps of: immunostaining the specimen with a fluorescent label; immobilizing the thus stained specimen; and mounting the thus immobilized specimen using a mounting medium including an organic solvent not freely miscible with water. In the biological substance detection method, the above-described mounting medium further includes a discoloration inhibitor.

公开(公告)号：US20190154582A1

公开(公告)日：2019-05-23

申请号：US16256224

申请日：2019-01-24

Applicant: Konica Minolta, Inc.

Inventor： Hideki Gouda ,  Kensaku Takanashi ,  Kohsuke Gonda ,  Noriaki Ohuchi ,  Mika Watanabe

IPC: G01N21/64 ,  G01N33/483 ,  G01N33/58 ,  G06T7/90 ,  G01N21/93 ,  G01N33/50 ,  G06T7/00 ,  G01N33/53 ,  G01N33/48

Abstract: A biological substance quantitation method includes the following. A fluorescent image is input, which represents an expression of a specific biological substance in a sample stained with a fluorescent substance by a florescent bright spot. A quantitative evaluation value of the florescent bright spot is calculated. A standard fluorescent image of a standard sample stained under a same condition as the sample and representing an expression of the biological substance in a standard sample, is input under a same condition as the fluorescent image. A quantitative evaluation value in the standard fluorescent image is calculated under a same condition as the fluorescent image. Based on a correlation between an expression amount of the biological substance in a standard sample measured in advance and the evaluation value in the standard fluorescent image, the evaluation value in the fluorescent image is converted to an expression amount of the biological substance in the sample.

公开(公告)号：US10234391B2

公开(公告)日：2019-03-19

申请号：US15549006

申请日：2015-02-10

Applicant: Konica Minolta, Inc.

Inventor： Hideki Gouda ,  Kensaku Takanashi ,  Kohsuke Gonda ,  Noriaki Ohuchi ,  Mika Watanabe

IPC: G06T7/00 ,  G06K9/00 ,  H04N7/18 ,  G01N21/64 ,  G01N33/48 ,  G01N33/53 ,  G06T7/90 ,  G01N21/93 ,  G01N33/50 ,  G01N33/58 ,  G01N33/483

Abstract: A biological substance quantitation method includes the following. A fluorescent image is input, which represents an expression of a specific biological substance in a sample stained with a fluorescent substance by a fluorescent bright spot. A quantitative evaluation value of the fluorescent bright spot is calculated. A standard fluorescent image of a standard sample stained under a same condition as the sample and representing an expression of the biological substance in a standard sample, is input under a same condition as the fluorescent image. A quantitative evaluation value in the standard fluorescent image is calculated under a same condition as the fluorescent image. Based on a correlation between an expression amount of the biological substance in a standard sample measured in advance and the evaluation value in the standard fluorescent image, the evaluation value in the fluorescent image is converted to an expression amount of the biological substance in the sample.

公开(公告)号：US11054417B2

公开(公告)日：2021-07-06

申请号：US14896831

申请日：2014-04-23

Applicant: Konica Minolta, Inc.

Inventor： Hideki Gouda ,  Masaru Takahashi ,  Kensaku Takanashi ,  Fuminori Okada

IPC: G01N33/543 ,  B82Y5/00 ,  B82Y15/00 ,  C09B67/00 ,  C09B67/08 ,  B82Y30/00 ,  B82Y40/00

Abstract: For fluorescent nanoparticles having a zeta potential of −10 mV to −60 mV at pH 7.0 or a zeta potential of 0 mV to −10 mV in a buffer of pH 6.0 to 8.0, an appropriate electrical repulsive force can be generated between biomolecules that are generally negatively charged and the fluorescent nanoparticles. As a result, non-specific binding between the fluorescent nanoparticles and the biomolecules is suppressed and the fluorescent nanoparticles are specifically bound to a biomolecule to be stained through interaction stronger than the electrical repulsive force, so that the visibility of the specific biomolecule to be stained can be improved. Further, since an appropriate electrical repulsive force is also generated between the fluorescent nanoparticles themselves, aggregation of the fluorescent nanoparticles can be inhibited and the dispersibility in a staining solution can thereby be maintained.

公开(公告)号：US20190033181A1

公开(公告)日：2019-01-31

申请号：US16150866

申请日：2018-10-03

Applicant: Konica Minolta, Inc.

Inventor： Kensaku TAKANASHI ,  Yasushi Nakano ,  Takeshi Isoda ,  Hideki Gouda

IPC: G01N1/30 ,  G01N33/52

Abstract: An object of the present invention is to provide: a staining agent for tissue staining which has an improved fluorescence signal evaluation accuracy; and a tissue staining kit comprising the staining agent. The staining agent for tissue staining contains, as a staining component, dye-resin particles comprising thermosetting resin particles and a fluorescent dye immobilized on the resin particles, wherein the resin particles contains a substituent having an electric charge opposite to that of the fluorescent dye and forms an ionic bond or a covalent bond with the fluorescent dye, and the dye-resin particles have a particle size variation coefficient of 15% or less.