公开(公告)号：US20060149042A1

公开(公告)日：2006-07-06

申请号：US10532998

申请日：2003-11-01

申请人： Konstantin Konstantinov ,  Huong Nguyen ,  Jens Vogel

发明人： Konstantin Konstantinov ,  Huong Nguyen ,  Jens Vogel

IPC分类号： C07K1/14

CPC分类号： C07K14/55 ,  C07K1/34 ,  C07K1/36

摘要： The invention provides methods for concentrating a macromolecule from a solution comprising the macromolecule and an organic polymer by first subjecting the solution to ultrafiltration to produce a first retentate solution, then adjusting the conductivity of the first retentate solution such that any protein precipitation induced by the organic polymer is essentially prevented to produce a second retentate solution, and then subjecting the second retentate solution to ultrafiltration. In a preferred embodiment, the conductivity is adjusted by diafiltration against water, suitable diluent or buffer. Preferably, the invention pertains to the concentration of solutions of native or recombinant proteins. The invention further pertains preferably to methods for the concentration of cell culture supernatant comprising a product protein and organic polymers of the Pluronic family of block co-polymers, and more preferably comprising Pluronic F-68 block co-polymer.

摘要翻译： 本发明提供了从包含大分子和有机聚合物的溶液中浓缩高分子的方法，首先使溶液经过超滤以产生第一滞留物溶液，然后调节第一渗余溶液的电导率，使得由有机物诱导的任何蛋白质沉淀 基本上防止聚合物产生第二滞留物溶液，然后使第二滞留物溶液经过超滤。 在优选的实施方案中，通过对水，合适的稀释剂或缓冲液进行渗滤来调节电导率。 优选地，本发明涉及天然或重组蛋白质的溶液的浓度。 本发明还优选地涉及浓缩包含产物蛋白质的细胞培养上清液和Pluronic族嵌段共聚物的有机聚合物，更优选包含Pluronic F-68嵌段共聚物的方法。

公开(公告)号：US06193883B1

公开(公告)日：2001-02-27

申请号：US08885308

申请日：1997-06-30

申请人： Karl-Heinz Kroner ,  Jens Vogel

发明人： Karl-Heinz Kroner ,  Jens Vogel

IPC分类号： B01D6508

CPC分类号： B01D65/08 ,  B01D29/01 ,  B01D29/018 ,  B01D29/54 ,  B01D29/86 ,  B01D2321/2008 ,  C12M47/02

摘要： A filtration apparatus for separating and/or enriching dissolved substances from a suspension or suspensions having at least one filter means through which flow takes place, there being arranged on the retentate side of the filter means a means for producing a shear field which produces a virtually homogeneous shear field and pressure gradient across the inflow cross-section of the filter means. The means for producing the shear field may be formed by a rotationally symmetrical rotor. There may be used as filter means matrices that are suitable for the adsorption of the dissolved substances to be separated off. There come into consideration for that purpose surface-modified membranes, for example affinity membranes. The filter apparatus may be one-tier, two-tier or multi-tier.

摘要翻译： 一种用于从具有至少一个过滤装置的悬浮液或悬浮液中分离和/或富集溶解物质的过滤装置，通过该过滤装置进行流动，在过滤器的滞留侧上设置有用于产生剪切场的装置， 过滤装置的流入横截面上的均匀剪切场和压力梯度。 用于产生剪切场的装置可以由旋转对称的转子形成。 可以使用适于吸附被分离的溶解物质的过滤装置的基质。 考虑到该目的的表面改性膜，例如亲和膜。 过滤装置可以是一层，二层或多层。