公开(公告)号：US20140315738A1

公开(公告)日：2014-10-23

申请号：US14256609

申请日：2014-04-18

Applicant: Korea Institute of Ocean Science & Technology

Inventor： Seungshic Yum ,  Seonock Woo ,  Hyokyoung Won ,  Aekyung Lee ,  Jae-Chun Ryu

IPC: C12Q1/68

CPC classification number: C12Q1/6883 ,  C12Q1/6876 ,  C12Q2600/142 ,  C12Q2600/158 ,  G01N33/743

Abstract: The present invention relates to marine medaka genes responding to the exposure of endocrine-disrupting chemicals, and a method for diagnosing an aquatic eco-system contamination using the marine medaka genes. Specifically, the method for diagnosing an aquatic eco-system contamination, according to the present invention, makes use of marine medaka genes of which the expression amount changes according to 17β-estradiol, or marine medaka genes of which the expression amount changes according to bisphenol A. Since it was verified that genes of marine medaka exposed to 17β-estradiol are changed or genes of marine medaka exposed to bisphenol A are changed, the present invention can be used in a useful manner for a microarray chip in which marine medaka genes responding to 17β-estradiol or marine medaka genes responding to bisphenol A are integrated, for a diagnosis using same, and for a method for diagnosing health or detecting stress of a marine eco-system by applying a kit having the microarray chip.

Abstract translation: 本发明涉及响应于内分泌干扰物质的暴露的海洋中间基因基因，以及使用海洋中间基因基因诊断水生生态系统污染的方法。 具体而言，根据本发明的水生生态系统污染的诊断方法利用表达量根据17＆bgr-雌二醇或表达量变化的海洋medaka基因的表达量变化的海洋medaka基因 双酚A。由于证实了暴露于17＆bgr-雌二醇的海洋中间药的基因发生变化或暴露于双酚A的海马medaka的基因发生了改变，所以本发明可以有用的方式用于其中海洋medaka的微阵列芯片 综合应用于双酚A的17＆bgr-雌二醇或海洋中间基因基因的基因，用于使用其的诊断，以及通过应用具有微阵列芯片的试剂盒来诊断健康或检测海洋生态系统的应力的方法。