公开(公告)号：US09248202B2

公开(公告)日：2016-02-02

申请号：US14463116

申请日：2014-08-19

Applicant: Korea University Research and Business Foundation ,  SFC CO., LTD.

Inventor： Bong-Rae Cho ,  Dong Eun Kang ,  Chang Su Lim ,  Ji Yeon Kim ,  Eun Sun Kim ,  Hoon Jai Chun

IPC: C07D405/14 ,  C12Q1/02 ,  A61K49/00 ,  C07D401/14

CPC classification number: A61K49/0052 ,  A61K49/0039 ,  C07D401/14 ,  C07D405/14 ,  C12Q1/02 ,  G01N33/57419

Abstract: The present disclosure relates to a two-photon probe for in-vivo imaging, having high selectivity for copper(II) ion, and a method for quantitatively estimating copper(II) on (Cu2+) in vivo using the same. The two-photon probe according to the present disclosure can detect intracellular free copper(II) on for a long period of time with a penetration depth greater than 90 μm in living cells and tissues without the problems of mistargeting and photobleaching and can quantitatively estimate copper(II) on concentration in vivo using the ratio of blue emission intensity at 400-450 nm and red emission intensity at 550-650 nm.

Abstract translation: 本公开内容涉及用于体内成像的双光子探针，其具有对铜（II）离子的高选择性，以及使用其对体内（Cu 2+）上的铜（II）进行定量估计的方法。 根据本公开的双光子探针可以在活细胞和组织中长时间检测细胞内游离铜（II），穿透深度大于90μm，而不会发生靶向和光漂白的问题，并且可以定量估计铜 （II）使用400-450nm的蓝色发射强度与550-650nm处的红色发射强度的比例。

公开(公告)号：US09220794B2

公开(公告)日：2015-12-29

申请号：US14459944

申请日：2014-08-14

Applicant: Korea University Research and Business Foundation

Inventor： Bong-Rae Cho ,  Chang Su Lim ,  Sajal Kumar Das ,  Sun Young Yang ,  Eun Sun Kim ,  Hoon Jai Chun

IPC: A61K49/00 ,  C07D277/66

CPC classification number: A61K49/0021 ,  C07D277/66

Abstract: The present disclosure relates to a two-photon fluorescent dye for imaging total sulfide in vivo and a method for quantitatively estimating total sulfide concentration in vivo using the same. The two-photon fluorescent dye, which includes a compound of [Chemical Formula 1], can detect hydrosulfide ion and image total sulfide and allows quantitative estimation of total sulfide concentration in vivo.

Abstract translation: 本公开内容涉及用于在体内成像总硫化物的双光子荧光染料和使用其同时量化体内总硫化物浓度的方法。 包含[化学式1]的化合物的双光子荧光染料可以检测氢硫化物离子和图像总硫化物，并允许定量估计体内总硫化物浓度。

公开(公告)号：US20150118162A1

公开(公告)日：2015-04-30

申请号：US14459944

申请日：2014-08-14

Applicant: Korea University Research and Business Foundation

Inventor： Bong-Rae Cho

IPC: A61K49/00

CPC classification number: A61K49/0021 ,  C07D277/66

Abstract: The present disclosure relates to a two-photon fluorescent dye for imaging total sulfide in vivo and a method for quantitatively estimating total sulfide concentration in vivo using the same. The two-photon fluorescent dye, which includes a compound of [Chemical Formula 1], can detect hydrosulfide on and image total sulfide and allows quantitative estimation of total sulfide concentration in vivo.

Abstract translation: 本公开内容涉及用于在体内成像总硫化物的双光子荧光染料和使用其同时量化体内总硫化物浓度的方法。 包括[化学式1]的化合物的双光子荧光染料可以检测氢硫化物并形成总硫化物，并允许定量估计体内总硫化物浓度。