公开(公告)号：US20240219308A1

公开(公告)日：2024-07-04

申请号：US17769601

申请日：2020-10-15

Applicant: Korea University Research and Business Foundation

Inventor： Ki Wan BONG ,  Yoohno ROH ,  Seok Joon MUN ,  Hyun Jee LEE ,  Ju Yeon KIM

IPC: G01N21/78 ,  G01N33/543 ,  G01N33/58

CPC classification number: G01N21/78 ,  G01N33/54313 ,  G01N33/581

Abstract: The present invention relates to a colorimetric detection of a target material based on a hydrogel particle. A colorimetric detection of a target material based on a hydrogel particle according to the embodiment of the present invention comprises the steps of: reacting a sample containing the target material with the hydrogel particle, which is loaded on the inside with a probe that specifically binds to the target material; and accumulating and amplifying an insoluble colorimetric material in the hydrogel particle so as to label the target material bound to the probe. Here, the hydrogel particle is made of a polymer network, the probe is loaded by binding to the polymer network, and the insoluble colorimetric material is fixed to the polymer network.

公开(公告)号：US20200309704A1

公开(公告)日：2020-10-01

申请号：US16954642

申请日：2018-12-14

Applicant: Korea University Research and Business Foundation

Inventor： Ki Wan BONG ,  Yoon Ho ROH ,  Hyun June MOON ,  Hyun Jee LEE ,  Hyeon Ung KIM ,  Seok Joon MUN

IPC: G01N21/64 ,  G01N21/71 ,  C12Q1/6834

Abstract: The present invention relates to a method for preparing encoded hydrogel particles for high sensitive detection of a target biomolecule with high accuracy, and encoded hydrogel particles prepared thereby and, specifically, to a method for preparing encoded hydrogel particles, comprising a step of synthesizing hydrogel particles, and then binding a probe thereto, and encoded hydrogel particles prepared thereby. According to the present invention, probes can be loaded with remarkably improved high efficiency, loaded probes can be uniformly distributed, and the potential problem of biomolecule detection inhibition caused by an unreacted end can be resolved. In addition, the present invention can be applied to the diagnosis of diseases or screening of drugs through high sensitive multiplex detection of target biomolecules such as nucleic acids and proteins, and therefore, can be widely used in the field of medical diagnosis including molecular diagnosis.