公开(公告)号：US11639491B2

公开(公告)日：2023-05-02

申请号：US16758947

申请日：2018-10-23

申请人： Kumiai Chemical Industry Co., Ltd. ,  K.I Chemical Industry Co., Ltd. ,  Okayama Prefectural Government

发明人： Toshinobu Yamazaki ,  Nobutoshi Myojo ,  Shingo Hattori ,  Tatsuya Horiuchi ,  Akira Kawaguchi ,  Namiko Kirino

IPC分类号： C12N1/00 ,  C12N1/04 ,  A01N63/20 ,  C12N1/20 ,  A61K35/74 ,  C12R1/41

摘要： Provided herein is a method for producing a lyophilized composition of viable cells of Rhizobium bacteria having high viable cell viability, and a lyophilized composition of viable cells of Rhizobium bacteria having desirable long-storage survivability, among others. The lyophilized composition of viable cells of Rhizobium bacteria having high viable cell viability and desirable long-storage survivability can be obtained when a composition containing viable cells of Rhizobium bacteria and more than 10 mass % moisture is lyophilized to bring the moisture content to 10 mass %, and the drying is ended before the moisture content becomes less than 5 mass % so that the composition after the lyophilization contains the viable bacteria and 5 to 10 mass % of moisture.

公开(公告)号：US11898139B2

公开(公告)日：2024-02-13

申请号：US16758950

申请日：2018-10-23

申请人： Kumiai Chemical Industry Co., Ltd. ,  K.I Chemical Industry Co., Ltd. ,  Okayama Prefectural Government

发明人： Toshinobu Yamazaki ,  Shingo Hattori ,  Tatsuya Horiuchi

IPC分类号： C12N1/20 ,  C12N1/04

CPC分类号： C12N1/20 ,  C12N1/04 ,  C12N2500/34 ,  C12N2500/60

摘要： Provided herein is a culturing method for bacteria of the genus Rhizobium whereby a low viscosity can be maintained in a culture solution during the culturing of and at the end of culturing of said bacteria, and that enables easy and efficient post-procedures, including concentration and separation of viable bacteria, among others. This is achieved by growing Rhizobium bacteria by liquid culture using a medium containing a disaccharide (for example, maltose, trehalose, lactose, sucrose) accounting for at least 25 mass % of the carbon source in the medium while maintaining a pH of 5.0 to 7.0 in the culture solution throughout the course of the culture, namely from the beginning of the culturing to the end of the culturing of said bacteria.