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1.发明授权Process for production of 5-ene-3-one or 3,6-dione derivatives of sterols, processes for production of lipid metabolism improvers, foods, drinks, and animal feeds, and analytical method 有权甾醇的5-烯-3-酮或3,6-二酮衍生物的制备方法，脂质代谢改进剂的生产方法，食品，饮料和动物饲料，以及分析方法公开(公告)号：US08008040B2
公开(公告)日：2011-08-30
申请号：US11632072
申请日：2005-07-12
IPC分类号： C12P33/00
CPC分类号： C12P33/00
摘要： An object of the present invention is to provide a process according to which 5-ene-3-one or 3,6-dione derivatives of sterols can be synthesized with a better yield, as well as a process for production of lipid metabolism improvers, foods, drinks, and animal feeds containing the 5-ene-3-one or 3,6-dione derivatives of sterols.The process for production of 5-ene-3-one or 3,6-dione derivatives of sterols of the present invention involves preparing a culturing solution containing an Arthrobacter bacterium, which is a microorganism exhibiting a cholesterol oxidase activity, and then, adding to the culturing solution an equivalent amount of hexane so as to prepare a bilayer solution consisting of an aqueous layer and a hydrocarbon-based solvent layer. Thereafter, sterol, or a derivative thereof, is made as a substrate contained therein, a reaction performed with cholesterol oxidase at 30° C. for 2 days, and, ethanol then added to separate a hydrocarbon-based solvent layer. Thereafter, the layer is dried to a state of dryness by means of a vacuum dryer so as to obtain 5-ene-3-one or 3,6-dione derivatives.
摘要翻译： 本发明的目的是提供可以以更好的产率合成甾醇的5-烯-3-酮或3,6-二酮衍生物的方法，以及脂质代谢改进剂的生产方法， 含有甾醇的5-烯-3-酮或3,6-二酮衍生物的食品，饮料和动物饲料。 制备本发明的甾醇的5-烯-3-酮或3,6-二酮衍生物的方法包括制备含有作为显示胆固醇氧化酶活性的微生物的节杆菌细菌的培养液，然后加入 培养液中加入等量的己烷，以制备由水层和烃基溶剂层组成的双层溶液。 此后，将固醇或其衍生物作为其中含有的底物，在30℃下用胆固醇氧化酶进行反应2天，然后加入乙醇以分离烃基溶剂层。 此后，通过真空干燥器将该层干燥至干燥状态，以获得5-烯-3-酮或3,6-二酮衍生物。
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2.发明申请Process for Production of 5-Ene-3-One or 3,6-Dione Derivatives of Sterols, Processes for Production of Lipid Metabolism Improvers, Foods, Drinks, and Animal Feeds, and Analytical Method 有权甾醇的5-烯-3-酮或3,6-二酮衍生物的制备方法，脂质代谢改良剂，食品，饮料和动物饲料的生产方法和分析方法公开(公告)号：US20080248520A1
公开(公告)日：2008-10-09
申请号：US11632072
申请日：2005-07-12
CPC分类号： C12P33/00
摘要： An object of the present invention is to provide a process according to which 5-ene-3-one or 3,6-dione derivatives of sterols can be synthesized with a better yield, as well as a process for production of lipid metabolism improvers, foods, drinks, and animal feeds containing the 5-ene-3-one or 3,6-dione derivatives of sterols.The process for production of 5-ene-3-one or 3,6-dione derivatives of sterols of the present invention involves preparing a culturing solution containing an Arthrobacter bacterium, which is a microorganism exhibiting a cholesterol oxidase activity, and then, adding to the culturing solution an equivalent amount of hexane so as to prepare a bilayer solution consisting of an aqueous layer and a hydrocarbon-based solvent layer. Thereafter, sterol, or a derivative thereof, is made as a substrate contained therein, a reaction performed with cholesterol oxidase at 30° C. for 2 days, and, ethanol then added to separate a hydrocarbon-based solvent layer. Thereafter, the layer is dried to a state of dryness by means of a vacuum dryer so as to obtain 5-ene-3-one or 3,6-dione derivatives.
摘要翻译： 本发明的目的是提供可以以更好的产率合成甾醇的5-烯-3-酮或3,6-二酮衍生物的方法，以及脂质代谢改进剂的生产方法， 含有甾醇的5-烯-3-酮或3,6-二酮衍生物的食品，饮料和动物饲料。 制备本发明的甾醇的5-烯-3-酮或3,6-二酮衍生物的方法包括制备含有节杆菌属细菌的培养液，其为显示胆固醇氧化酶活性的微生物，然后加入 培养液中加入等量的己烷，以制备由水层和烃基溶剂层组成的双层溶液。 此后，将固醇或其衍生物作为其中含有的底物，在30℃下用胆固醇氧化酶进行反应2天，然后加入乙醇以分离烃基溶剂层。 此后，通过真空干燥器将该层干燥至干燥状态，以获得5-烯-3-酮或3,6-二酮衍生物。
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