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公开(公告)号：US10823951B2
公开(公告)日：2020-11-03
申请号：US16326937
申请日：2017-08-17
摘要： A method for imaging in a microscope with oblique illumination includes illuminating an object by an illumination beam path that is obliquely incident on an object plane of the microscope. A microscopic image of the object and a corresponding digital image signal are produced. The digital image signal is processed by digital image processing using a convolution kernel to increase contrast. An increased-contrast digital image is produced from the processed digital image signal.
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公开(公告)号：US20200319445A1
公开(公告)日：2020-10-08
申请号：US16300966
申请日：2017-05-12
摘要： An optical scanning microscope includes an illumination system having a light source portion emanating from a light source, first and second polarizing beam splitters, and first and second optical channels disposed between the beam splitters. The light source portion is configured to emit a first illumination light beam comprising light of a first and of a second main polarization direction. The first beam splitter is configured to guide the light of the first and second main polarization direction primarily into the first and channels, respectively. The second beam splitter is configured to form a second illumination light beam from light of the first and second main polarization directions from the first channel and second channels, respectively. The first and second channels are configured to emit the light of the first and second main polarization directions from the first and second channels, respectively, so as to have different convergence angles.
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公开(公告)号：US11630292B2
公开(公告)日：2023-04-18
申请号：US16300966
申请日：2017-05-12
摘要： An optical scanning microscope includes an illumination system having a light source portion emanating from a light source, first and second polarizing beam splitters, and first and second optical channels disposed between the beam splitters. The light source portion is configured to emit a first illumination light beam comprising light of a first main polarization direction and of a second main polarization direction. The first beam splitter is configured to guide the light primarily into the first and channels, respectively. The second beam splitter is configured to form a second illumination light beam from light of the first and second main polarization directions from the first channel and second channels, respectively. The first and second channels are configured to emit the light of the first and second main polarization directions from the first and second channels, respectively, so as to have different types of convergence.
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公开(公告)号：US20190391380A1
公开(公告)日：2019-12-26
申请号：US16326937
申请日：2017-08-17
摘要： A method for imaging in a microscope with oblique illumination includes illuminating an object by an illumination beam path that is obliquely incident on an object plane of the microscope. A microscopic image of the object and a corresponding digital image signal are produced. The digital image signal is processed by digital image processing using a convolution kernel to increase contrast. An increased-contrast digital image is produced from the processed digital image signal.
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公开(公告)号：US09042012B2
公开(公告)日：2015-05-26
申请号：US13649243
申请日：2012-10-11
发明人： Albrecht Weiss
CPC分类号： G02B21/086 , G02B27/141
摘要： Illuminating arrangement for a microscope (200) having a first LED (10) for providing light with a first intensity spectrum (K1) with at least two intensity maxima and an intensity minimum located between the intensity maxima, and at least one further LED (20) for providing light with a further intensity spectrum (K2) respectively, each further intensity spectrum (K2) having an intensity maximum in the region of the intensity minimum of the first intensity spectrum (K1), and a device (30) for merging the light of the first LED (10) and the light of the at least one further LED (20), by means of which illuminating light can be produced with a combined intensity spectrum (K3) composed of light with the first intensity spectrum and light with each of the further intensity spectra.
摘要翻译： 具有第一LED（10）的照明装置，所述第一LED（10）用于向光提供具有至少两个强度最大值的第一强度光谱（K1）和位于强度最大值之间的强度最小值以及至少一个另外的LED（20 ），用于分别提供具有另一强度光谱（K2）的光，在所述第一强度光谱（K1）的强度最小值的区域中具有强度最大值的每个另外的强度光谱（K2）和用于将所述第 第一LED（10）的光和至少一个另外的LED（20）的光，借助于该照明光可以利用由具有第一强度光谱的光组成的组合强度光谱（K3）和具有 每个进一步的强度光谱。
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