公开(公告)号：US20240361584A1

公开(公告)日：2024-10-31

申请号：US18645424

申请日：2024-04-25

申请人： LEICA MICROSYSTEMS CMS GMBH ,  BioAxial SAS

发明人： Gabriel SIRAT ,  Tom SPERBER ,  Lionel MOISAN ,  Marcus DYBA

IPC分类号： G02B21/06 ,  G01N15/1434 ,  G02B21/16 ,  G02B21/36

CPC分类号： G02B21/06 ,  G01N15/1434 ,  G02B21/16 ,  G02B21/365

摘要： A single-particle localization microscope includes a light source configured to generate illumination light for illuminating a sample region, and an optical illumination system configured to shape the illumination light into a localizing light distribution having a substantially zero intensity minimum at a target point within the sample region. The localizing light distribution is adapted to cause a single particle in a fluorescent state located in the sample region outside the intensity minimum to emit fluorescent light. The optical illumination system is further configured to shape the illumination light into an auxiliary light distribution having a non-zero intensity at the target point such that the auxiliary light distribution is defined in a spatial extent and/or in its shape by the localizing light distribution.