公开(公告)号：US20230295605A1

公开(公告)日：2023-09-21

申请号：US18069171

申请日：2022-12-20

Applicant: LIFE TECHNOLOGIES AS

Inventor： Laure JOBERT

IPC: C12N15/10

CPC classification number: C12N15/1013

Abstract: This invention relates to methods and compositions for coupling nucleic acid to a functionalized surface or support. In particular, the present invention provides an improved process for coupling aminated nucleic acid to a support functionalized with carboxylic acid groups, wherein the coupling reaction is conducted in the presence of an organic solvent. The invention further relates to compositions and kits for performing the coupling reaction and uses of nucleic acid-loaded supports for various applications.

公开(公告)号：US20230407288A1

公开(公告)日：2023-12-21

申请号：US18252283

申请日：2021-11-10

Applicant: LIFE TECHNOLOGIES AS

Inventor： Laure JOBERT ,  Marie BOSNES ,  Hannah Kim LINDSTROM

IPC: C12N15/10 ,  C12P19/34

CPC classification number: C12N15/1013 ,  C12P19/34

Abstract: A method of separating RNA from a sample, comprising providing: a sample comprising RNA, a binding solution comprising an oligoethylene glycol and a salt, and a solid support having a hydrophilic surface. The method further comprises contacting the sample with the binding solution and solid support, under conditions that allow binding of the RNA in the sample to the surface of the solid support, thereby providing a solid support with bound RNA in contact with residual solution; and separating the solid support with bound RNA from the residual solution. During the binding of the RNA to the surface of the solid support, oligoethylene glycol is present at a concentration of at least about 35% v/v and the salt is present at a concentration of between about 1 M and about 2 M. Methods for producing purified RNA are also provided, comprising in vitro transcription to produce an RNA molecule with a first magnetic bead and purification with a second magnetic bead. Also provided are kits and uses.

公开(公告)号：US20200277595A1

公开(公告)日：2020-09-03

申请号：US16645896

申请日：2018-08-30

Applicant: LIFE TECHNOLOGIES AS

Inventor： Laure JOBERT

IPC: C12N15/10

Abstract: This invention relates to methods and compositions for coupling nucleic acid to a functionalized surface or support. In particular, the present invention provides an improved process for coupling aminated nucleic acid to a support functionalized with carboxylic acid groups, wherein the coupling reaction is conducted in the presence of an organic solvent. The invention further relates to compositions and kits for performing the coupling reaction and uses of nucleic acid-loaded supports for various applications.