公开(公告)号：US20240392226A1

公开(公告)日：2024-11-28

申请号：US18248428

申请日：2021-10-12

Applicant: LIFE TECHNOLOGIES AS ,  LIFE TECHNOLOGIES CORPORATION ,  LIFE TECHNOLOGIES HOLDINGS PTE LIMITED

Inventor： Morten LUHR ,  Michael GORDON ,  Ming Tiong SIA ,  Kok Shyong CHONG ,  Woon Liang Terence SOH ,  Seng Leong LIM ,  Ida Caroline SCHROEDER ,  Katrine ALFSNES ,  Terry JianHui LIM ,  Marie BOSNES ,  Axl Alois NEURAUTER ,  Ketil Winther PEDERSEN ,  Autumn Day WILBUR ,  Sean ZIMMERMAN

IPC: C12M1/12 ,  C12M1/00 ,  G01N35/00

Abstract: A bead processing assembly for use in attaching magnetic beads to biological cells and other biological materials and/or separating magnetic beads from biological cells and other biological materials includes a base assembly having a housing, a support panel disposed on the housing and having a front face, a first pinch valve at least partially outwardly projecting from the front face of the support panel, and a first pump at least partially outwardly projecting from the front face of the support panel. A rocker assembly is supported on the base assembly and includes a mount assembly supported on the base assembly, a platform assembly pivotably secured to the mount assembly, and a rocker drive for repeatedly rocking the platform assembly relative to the mount assembly.

公开(公告)号：US20230407288A1

公开(公告)日：2023-12-21

申请号：US18252283

申请日：2021-11-10

Applicant: LIFE TECHNOLOGIES AS

Inventor： Laure JOBERT ,  Marie BOSNES ,  Hannah Kim LINDSTROM

IPC: C12N15/10 ,  C12P19/34

CPC classification number: C12N15/1013 ,  C12P19/34

Abstract: A method of separating RNA from a sample, comprising providing: a sample comprising RNA, a binding solution comprising an oligoethylene glycol and a salt, and a solid support having a hydrophilic surface. The method further comprises contacting the sample with the binding solution and solid support, under conditions that allow binding of the RNA in the sample to the surface of the solid support, thereby providing a solid support with bound RNA in contact with residual solution; and separating the solid support with bound RNA from the residual solution. During the binding of the RNA to the surface of the solid support, oligoethylene glycol is present at a concentration of at least about 35% v/v and the salt is present at a concentration of between about 1 M and about 2 M. Methods for producing purified RNA are also provided, comprising in vitro transcription to produce an RNA molecule with a first magnetic bead and purification with a second magnetic bead. Also provided are kits and uses.