公开(公告)号：US20190323091A1

公开(公告)日：2019-10-24

申请号：US16503262

申请日：2019-07-03

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Kelli BRAMLETT ,  Dumitru BRINZA ,  Richard CHIEN ,  Dalia DHINGRA ,  Jian GU ,  Ann MONGAN

IPC: C12Q1/6886 ,  C12Q1/6827 ,  C12Q1/6874

Abstract: In some embodiments, the disclosure relates generally to methods, as well as related systems, compositions, kits, apparatuses and computer-readable media, comprising a multiplex molecular tagging procedure that employs a plurality of tags that are appended to a plurality of polynucleotides. The tags have characteristics, including a sequence, length and/or detectable moiety, or any other characteristic, that uniquely identifies the polynucleotide molecule to which it is appended, and permits tracking individual tagged molecules in a mixture of tagged molecules. For example, the tag having a unique tag sequence, can uniquely identify an individual polynucleotide to which it is appended, and distinguish the individual polynucleotide from other tagged polynucleotides in a mixture. In some embodiments, the multiplex molecular tagging procedure can be used for generating error-corrected sequencing data and for detecting a target polynucleotide which is present at low abundance in a nucleic acid sample.

公开(公告)号：US20210363596A1

公开(公告)日：2021-11-25

申请号：US17389009

申请日：2021-07-29

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Kelli BRAMLETT ,  Dumitru BRINZA ,  Richard CHIEN ,  Dalia DHINGRA ,  Jian GU ,  Ann MONGAN

IPC: C12Q1/6886 ,  C12Q1/6827 ,  C12Q1/6874

Abstract: In some embodiments, the disclosure relates generally to methods, as well as related systems, compositions, kits, apparatuses and computer-readable media, comprising a multiplex molecular tagging procedure that employs a plurality of tags that are appended to a plurality of polynucleotides. The tags have characteristics, including a sequence, length and/or detectable moiety, or any other characteristic, that uniquely identifies the polynucleotide molecule to which it is appended, and permits tracking individual tagged molecules in a mixture of tagged molecules. For example, the tag having a unique tag sequence, can uniquely identify an individual polynucleotide to which it is appended, and distinguish the individual polynucleotide from other tagged polynucleotides in a mixture. In some embodiments, the multiplex molecular tagging procedure can be used for generating error-corrected sequencing data and for detecting a target polynucleotide which is present at low abundance in a nucleic acid sample.