公开(公告)号：US20180330047A1

公开(公告)日：2018-11-15

申请号：US15967501

申请日：2018-04-30

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Harrison Leong ,  Nivedita Sumi Majumdar ,  Elana E Swartzman

IPC: G06F19/18 ,  G06F19/24

CPC classification number: G06F19/18 ,  G06F19/24

Abstract: A proximity binding assay (PBA) is performed on at least one test sample, at least one reference sample, a background sample, and one or more calibration samples using a thermal cycler instrument. Ct values are determined for at least one set of test sample data and at least one set of reference sample data. Background corrected Ct values are calculated using a corresponding value in a background sample data set. A linear range is determined for the background corrected Ct values as a function of sample quantity. A linear regression line is calculated for each linear range. One or more parameter values of an exponential model (EM) fold change formula are estimated from the one or more sets of calibration sample data. A target protein quantity and associated confidence interval are calculated using the linear regression lines and the EM fold change formula.