公开(公告)号：US20140363854A1

公开(公告)日：2014-12-11

申请号：US14272433

申请日：2014-05-07

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Michael D. SMITH ,  Robert Jason Potter ,  Gulshan Dhariwal ,  Gary F. Gerard ,  Kim Rosenthal ,  Jun E. Lee

IPC: C12N9/12 ,  C12P19/34

CPC classification number: C12N9/1276 ,  C12P19/34 ,  C12Y207/07049

Abstract: The present invention is in the fields of molecular and cellular biology. The invention is generally related to reverse transcriptase enzymes and methods for the reverse transcription of nucleic acid molecules, especially messenger RNA molecules. Specifically, the invention relates to reverse transcriptase enzymes which have been mutated or modified to increase thermostability, decrease terminal deoxynucleotidyl transferase activity, and/or increase fidelity, and to methods of producing, amplifying or sequencing nucleic acid molecules (particularly cDNA molecules) using these reverse transcriptase enzymes or compositions. The invention also relates to nucleic acid molecules produced by these methods and to the use of such nucleic acid molecules to produce desired polypeptides. The invention also concerns kits comprising such enzymes or compositions.

Abstract translation: 本发明涉及分子和细胞生物学领域。 本发明通常涉及逆转录酶和逆转录核酸分子，特别是信使RNA分子的方法。 具体地，本发明涉及已被突变或修饰以增加热稳定性，降低末端脱氧核苷酸转移酶活性和/或增加保真度的逆转录酶，以及使用这些方法来产生，扩增或测序核酸分子（特别是cDNA分子）的方法 逆转录酶或组合物。 本发明还涉及通过这些方法产生的核酸分子以及这种核酸分子用于产生所需多肽的用途。 本发明还涉及包含这些酶或组合物的试剂盒。