公开(公告)号：US09862938B2

公开(公告)日：2018-01-09

申请号：US14272433

申请日：2014-05-07

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Michael D. Smith ,  Robert Jason Potter ,  Gulshan Dhariwal ,  Gary F. Gerard ,  Kim Rosenthal ,  Jun E. Lee

IPC: C12N9/12 ,  C12P19/34

CPC classification number: C12N9/1276 ,  C12P19/34 ,  C12Y207/07049

Abstract: The present invention is in the fields of molecular and cellular biology. The invention is generally related to reverse transcriptase enzymes and methods for the reverse transcription of nucleic acid molecules, especially messenger RNA molecules. Specifically, the invention relates to reverse transcriptase enzymes which have been mutated or modified to increase thermostability, decrease terminal deoxynucleotidyl transferase activity, and/or increase fidelity, and to methods of producing, amplifying or sequencing nucleic acid molecules (particularly cDNA molecules) using these reverse transcriptase enzymes or compositions. The invention also relates to nucleic acid molecules produced by these methods and to the use of such nucleic acid molecules to produce desired polypeptides. The invention also concerns kits comprising such enzymes or compositions.