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    METHODS, SYSTEMS, AND COMPUTER READABLE MEDIA FOR REPEAT SEQUENCING
    1.
    发明申请
    METHODS, SYSTEMS, AND COMPUTER READABLE MEDIA FOR REPEAT SEQUENCING 审中-公开

    公开(公告)号：US20200024656A1

    公开(公告)日：2020-01-23

    申请号：US16429266

    申请日：2019-06-03

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Earl Hubbell ,  Christian Koller ,  Nils Homer

    IPC: C12Q1/6869

    Abstract: A method for sequencing a nucleic acid template includes: (a) performing a first sequencing process including flowing nucleotides and/or reagents to the nucleic acid template according to a first predetermined ordering of nucleotides and/or reagents to obtain a first sequencing result; (b) after the first sequencing process, performing a second sequencing process including flowing nucleotides and/or reagents to the nucleic acid template according to a second predetermined ordering of nucleotides and/or reagents to obtain a second sequencing result, the second predetermined ordering of nucleotides and/or reagents being different from the first predetermined ordering of nucleotides and/or reagents and at least one of the first and second predetermined orderings of nucleotides and/or reagents being designed for repeat sequencing; and (c) determining a sequence of bases corresponding to at least a portion of the nucleic acid template using both the first sequencing result and the second sequencing result.

    Methods, systems, and computer readable media for repeat sequencing
    3.
    发明授权
    Methods, systems, and computer readable media for repeat sequencing 有权

    公开(公告)号：US11655500B2

    公开(公告)日：2023-05-23

    申请号：US16429266

    申请日：2019-06-03

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Earl Hubbell ,  Christian Koller ,  Nils Homer

    IPC: C12Q1/6869 ,  G16B30/00

    CPC classification number: C12Q1/6869 ,  G16B30/00 ,  C12Q2535/122 ,  C12Q1/6869 ,  C12Q2525/179 ,  C12Q2535/119 ,  C12Q2537/165

    Abstract: A method for sequencing a nucleic acid template includes: (a) performing a first sequencing process including flowing nucleotides and/or reagents to the nucleic acid template according to a first predetermined ordering of nucleotides and/or reagents to obtain a first sequencing result; (b) after the first sequencing process, performing a second sequencing process including flowing nucleotides and/or reagents to the nucleic acid template according to a second predetermined ordering of nucleotides and/or reagents to obtain a second sequencing result, the second predetermined ordering of nucleotides and/or reagents being different from the first predetermined ordering of nucleotides and/or reagents and at least one of the first and second predetermined orderings of nucleotides and/or reagents being designed for repeat sequencing; and (c) determining a sequence of bases corresponding to at least a portion of the nucleic acid template using both the first sequencing result and the second sequencing result.

    Methods and Systems for Nucleic Acid Sequence Analysis
    5.
    发明申请
    Methods and Systems for Nucleic Acid Sequence Analysis 有权
    核酸序列分析方法与系统

    公开(公告)号：US20140220558A1

    公开(公告)日：2014-08-07

    申请号：US14055520

    申请日：2013-10-16

    Applicant: Life Technologies Corporation

    Inventor： Nils Homer

    IPC: G06F19/22

    CPC classification number: G06F19/22 ,  C12Q1/6869 ,  G06F19/24 ,  C12Q2527/119 ,  C12Q2533/101 ,  C12Q2565/607

    Abstract: Disclosed are new and improved methods and systems for nucleic acid sequence analysis that can analyze data indicative of natural by-products of nucleotide incorporation events without the need for exogenous labels or dyes to identify nucleic acid sequences of interest. In particular, the methods and systems of the present teachings can process such data and various forms thereof to align fragments of the nucleic acid(s) of interest, particularly those analyzed using an addition sequencing technique, for example, as occurs with the use of nucleotide flows.

    Abstract translation: 公开了用于核酸序列分析的新的和改进的方法和系统，其可以分析指示核苷酸掺入事件的天然副产物的数据，而不需要外源标记或染料来鉴定感兴趣的核酸序列。 特别地，本教导的方法和系统可以处理这些数据及其各种形式，以使目标核酸的片段，特别是使用加成测序技术分析的那些核酸片段，例如，如使用 核苷酸流。

    Methods, systems, and computer readable media for repeat sequencing
    6.
    发明授权
    Methods, systems, and computer readable media for repeat sequencing 有权

    公开(公告)号：US12077818B2

    公开(公告)日：2024-09-03

    申请号：US18297049

    申请日：2023-04-07

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Earl Hubbell ,  Christian Koller ,  Nils Homer

    IPC: C12Q1/6869 ,  G16B30/00

    CPC classification number: C12Q1/6869 ,  G16B30/00 ,  C12Q2535/122 ,  C12Q1/6869 ,  C12Q2525/179 ,  C12Q2535/119 ,  C12Q2537/165

    Abstract: A method for sequencing a nucleic acid template includes: (a) performing a first sequencing process including flowing nucleotides and/or reagents to the nucleic acid template according to a first predetermined ordering of nucleotides and/or reagents to obtain a first sequencing result; (b) after the first sequencing process, performing a second sequencing process including flowing nucleotides and/or reagents to the nucleic acid template according to a second predetermined ordering of nucleotides and/or reagents to obtain a second sequencing result, the second predetermined ordering of nucleotides and/or reagents being different from the first predetermined ordering of nucleotides and/or reagents and at least one of the first and second predetermined orderings of nucleotides and/or reagents being designed for repeat sequencing; and (c) determining a sequence of bases corresponding to at least a portion of the nucleic acid template using both the first sequencing result and the second sequencing result.

    METHODS, SYSTEMS, AND COMPUTER READABLE MEDIA FOR REPEAT SEQUENCING
    7.
    发明公开
    METHODS, SYSTEMS, AND COMPUTER READABLE MEDIA FOR REPEAT SEQUENCING 审中-公开

    公开(公告)号：US20230348966A1

    公开(公告)日：2023-11-02

    申请号：US18297049

    申请日：2023-04-07

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Earl Hubbell ,  Christian Koller ,  Nils Homer

    IPC: C12Q1/6869 ,  G16B30/00

    CPC classification number: C12Q1/6869 ,  G16B30/00 ,  C12Q2535/122

    Abstract: A method for sequencing a nucleic acid template includes: (a) performing a first sequencing process including flowing nucleotides and/or reagents to the nucleic acid template according to a first predetermined ordering of nucleotides and/or reagents to obtain a first sequencing result; (b) after the first sequencing process, performing a second sequencing process including flowing nucleotides and/or reagents to the nucleic acid template according to a second predetermined ordering of nucleotides and/or reagents to obtain a second sequencing result, the second predetermined ordering of nucleotides and/or reagents being different from the first predetermined ordering of nucleotides and/or reagents and at least one of the first and second predetermined orderings of nucleotides and/or reagents being designed for repeat sequencing; and (c) determining a sequence of bases corresponding to at least a portion of the nucleic acid template using both the first sequencing result and the second sequencing result.

    Methods, systems, and computer readable media for repeat sequencing
    9.
    发明授权
    Methods, systems, and computer readable media for repeat sequencing 有权

    公开(公告)号：US10329608B2

    公开(公告)日：2019-06-25

    申请号：US14048843

    申请日：2013-10-08

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Earl Hubbell ,  Christian Koller ,  Nils Homer

    IPC: C12Q1/6869

    Abstract: A method for sequencing a nucleic acid template includes: (a) performing a first sequencing process including flowing nucleotides and/or reagents to the nucleic acid template according to a first predetermined ordering of nucleotides and/or reagents to obtain a first sequencing result; (b) after the first sequencing process, performing a second sequencing process including flowing nucleotides and/or reagents to the nucleic acid template according to a second predetermined ordering of nucleotides and/or reagents to obtain a second sequencing result, the second predetermined ordering of nucleotides and/or reagents being different from the first predetermined ordering of nucleotides and/or reagents and at least one of the first and second predetermined orderings of nucleotides and/or reagents being designed for repeat sequencing; and (c) determining a sequence of bases corresponding to at least a portion of the nucleic acid template using both the first sequencing result and the second sequencing result.

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