公开(公告)号：US20150275257A1

公开(公告)日：2015-10-01

申请号：US14622653

申请日：2015-02-13

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Mark G. Erlander ,  Ranelle C. Salunga ,  Steven T. Kunitake ,  Liang You ,  Theresa B. Taylor

IPC: C12P19/34

CPC classification number: C12P19/34 ,  C12N15/1096 ,  C12Q1/6846 ,  C12Q2525/179 ,  C12Q2525/143 ,  C12Q2521/107

Abstract: The present invention provides improved methods for the amplification of nucleic acid molecules. Methods for amplifying target polynucleotides, including mRNA, using oligonucleotides, DNA and RNA polymerases are provided. The invention further provides compositions and kits for practicing the methods, as well as methods which use the amplification products.

Abstract translation: 本发明提供了用于扩增核酸分子的改进方法。 提供了使用寡核苷酸，DNA和RNA聚合酶扩增靶多核苷酸（包括mRNA）的方法。 本发明还提供了用于实施该方法的组合物和试剂盒，以及使用扩增产物的方法。