公开(公告)号：US3497546A

公开(公告)日：1970-02-24

申请号：US3497546D

申请日：1966-11-28

Applicant: LITTLE INC A

Inventor： LEVINS PHILIP L ,  JOHNSON DENNIS E ,  KRANICH WILMER L

IPC: C07C69/38 ,  C07C121/22 ,  C07C121/02

CPC classification number: C07C255/00

公开(公告)号：US3530658A

公开(公告)日：1970-09-29

申请号：US3530658D

申请日：1969-11-17

Applicant: LITTLE INC A

Inventor： JOHNSON DENNIS E

IPC: D01H1/10

CPC classification number: D01H1/101

公开(公告)号：US3647632A

公开(公告)日：1972-03-07

申请号：US3647632D

申请日：1968-04-11

Applicant: LITTLE INC A

Inventor： JOHNSON DENNIS E ,  WOODLAND LAWRENCE R ,  KENSLER CHARLES J ,  HIMMELFARB PHILIP

IPC: C12M1/06 ,  C12M1/12 ,  C12M1/26 ,  C12M3/06 ,  C12B1/10

CPC classification number: C12M29/04 ,  C12M27/02

Abstract: METHOD AND APPARATUS FOR HANDLING CELLS WHICH MAY BE MICROORGANISMS OR MAY BE DERIVED FROM THE TISSUES OF LIVING ORGANISMS. THE METHOD AND APPARATUS PERMIT GROWING SUCH CELLS IN AN ACTIVE GROWTH STATE TO POPULATION DENSITIES WHICH MAY BE FROM 2 TO 35 TIMES THE DENSITIES NOW POSSIBLE WITH PRESENT METHODS AND EQUIPMENT. THE CELLS ARE MAINTAINED SUSPENDED IN A LIQUID MEDIUM IN WHICH IS PLACED A ROTATING FILTER. LIQUIDS MAY BE INTRODUCED AND WITHDRAWN WITHOUT DISTRUBING THE CELLS. CELL DENSITIES MAY BE BUILT UP TO A HIGH LEVEL AND USED AS MODEL TISSUES TO DETERMINE THE EFFECT OF CHEMICAL AGENTS ON THEM UNDER CONDITIONS WHICH ARE SIMILAR TO THOSE IN LIVING ORGANISMS. THE METHOD AND APPARATUS ARE ALSO SUITABLE FOR CARRYING OUT LARGE-SCALE INDUSTRIAL PROCESS INCLUDING THE SYNTHESIS OF ORGANIC CHEMICALS AS WELL AS THE TREATMENT OF SEWAGE.

公开(公告)号：US3541133A

公开(公告)日：1970-11-17

申请号：US3541133D

申请日：1969-04-03

Applicant: LITTLE INC A

Inventor： JOHNSON DENNIS E ,  LEVINE PHILIP L ,  KRANICH WILMER L

IPC: C07C121/22

CPC classification number: C07C255/00