公开(公告)号：US12077786B2

公开(公告)日：2024-09-03

申请号：US16966768

申请日：2019-02-01

申请人： LONZA LTD

发明人： Robert Young ,  Christopher Mark Smales ,  Colin Mark Jaques ,  Andrew J. Racher ,  Gurdeep Singh ,  James Budge ,  Joanne Robool

IPC分类号： C12N9/06 ,  C12N9/02 ,  C12N15/10 ,  C12N15/63

CPC分类号： C12N9/0008 ,  C12N15/1075 ,  C12N15/63

摘要： Described herein are compositions and methods for identifying, selecting, or culturing cells comprising a subject nucleic acid sequence of interest. Generally, a nucleic acid comprising a subject nucleic acid and a sequence encoding an enzyme molecule involved in biosynthesis of an amino acid is introduced into a cell. The cell is then grown on media lacking the amino acid, such that cells comprising the introduced nucleic acid are capable of growth. In some instances, the cell further comprises an inhibitor of the enzyme molecule to increase the stringency of the selection.