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公开(公告)号：US12077786B2
公开(公告)日：2024-09-03
申请号：US16966768
申请日：2019-02-01
申请人： LONZA LTD
发明人： Robert Young , Christopher Mark Smales , Colin Mark Jaques , Andrew J. Racher , Gurdeep Singh , James Budge , Joanne Robool
CPC分类号： C12N9/0008 , C12N15/1075 , C12N15/63
摘要： Described herein are compositions and methods for identifying, selecting, or culturing cells comprising a subject nucleic acid sequence of interest. Generally, a nucleic acid comprising a subject nucleic acid and a sequence encoding an enzyme molecule involved in biosynthesis of an amino acid is introduced into a cell. The cell is then grown on media lacking the amino acid, such that cells comprising the introduced nucleic acid are capable of growth. In some instances, the cell further comprises an inhibitor of the enzyme molecule to increase the stringency of the selection.
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公开(公告)号：US20190120824A1
公开(公告)日：2019-04-25
申请号：US16068422
申请日：2017-01-06
申请人： LONZA LTD
摘要： Disclosed herein are methods and compositions useful for evaluating, selecting, identifying, or making a cell or cell line that has improved production capacity for generating higher yields of products and/or improved capacity to produce higher quality products. Products, as described herein, can include a polypeptide that is endogenously expressed by the cell, a recombinant polypeptide that is not endogenously expressed, or a non-naturally occurring recombinant polypeptide. The methods described herein include modulating, e.g., inhibiting, the protein degradation pathway by using a proteasome inhibitor, an ER-associated degradation (ERAD) inhibitor, or a ubiquitin pathway inhibitor.
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公开(公告)号：US20200231944A1
公开(公告)日：2020-07-23
申请号：US16818162
申请日：2020-03-13
申请人： LONZA LTD
摘要： The present disclosure features methods and compositions for modulating lipid metabolism to achieve improved production and quality of recombinant products, such as next generation biologics. Modulation of lipid metabolism as described herein includes, for example, introducing a lipid metabolism modulator described herein to a cell or a cell-free system. Also encompassed by the present disclosure are engineered cells with improved production capacity and improved product quality, methods for engineering such cells, and preparations and mixtures comprising the products from such cells.
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公开(公告)号：US11320422B2
公开(公告)日：2022-05-03
申请号：US16068422
申请日：2017-01-06
申请人： LONZA LTD
摘要： Disclosed herein are methods and compositions useful for evaluating, selecting, identifying, or making a cell or cell line that has improved production capacity for generating higher yields of products and/or improved capacity to produce higher quality products. Products, as described herein, can include a polypeptide that is endogenously expressed by the cell, a recombinant polypeptide that is not endogenously expressed, or a non-naturally occurring recombinant polypeptide. The methods described herein include modulating, e.g., inhibiting, the protein degradation pathway by using a proteasome inhibitor, an ER-associated degradation (ERAD) inhibitor, or a ubiquitin pathway inhibitor.
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公开(公告)号：US20170321194A1
公开(公告)日：2017-11-09
申请号：US15585570
申请日：2017-05-03
申请人： LONZA LTD
CPC分类号： C12N9/0097 , C07K14/4702 , C07K16/00 , C07K2317/14 , C12N5/0682 , C12N9/0071 , C12N9/0083 , C12P21/02 , C12Y114/19001
摘要： The present disclosure features methods and compositions for modulating lipid metabolism to achieve improved production and quality of recombinant products, such as next generation biologics. Modulation of lipid metabolism as described herein includes, for example, introducing a lipid metabolism modulator described herein to a cell or a cell-free system. Also encompassed by the present disclosure are engineered cells with improved production capacity and improved product quality, methods for engineering such cells, and preparations and mixtures comprising the products from such cells.
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公开(公告)号：US11434474B2
公开(公告)日：2022-09-06
申请号：US16818162
申请日：2020-03-13
申请人： LONZA LTD
摘要： The present disclosure features methods and compositions for modulating lipid metabolism to achieve improved production and quality of recombinant products, such as next generation biologics. Modulation of lipid metabolism as described herein includes, for example, introducing a lipid metabolism modulator described herein to a cell or a cell-free system. Also encompassed by the present disclosure are engineered cells with improved production capacity and improved product quality, methods for engineering such cells, and preparations and mixtures comprising the products from such cells.
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公开(公告)号：US10655111B2
公开(公告)日：2020-05-19
申请号：US15585570
申请日：2017-05-03
申请人： LONZA LTD
摘要： The present disclosure features methods and compositions for modulating lipid metabolism to achieve improved production and quality of recombinant products, such as next generation biologics. Modulation of lipid metabolism as described herein includes, for example, introducing a lipid metabolism modulator described herein to a cell or a cell-free system. Also encompassed by the present disclosure are engineered cells with improved production capacity and improved product quality, methods for engineering such cells, and preparations and mixtures comprising the products from such cells.
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