公开(公告)号：US20230313256A1

公开(公告)日：2023-10-05

申请号：US18003991

申请日：2021-07-01

申请人： LONZA LTD

发明人： Nuria ADELANTADO ,  Hauke HOLM ,  Christoph KIZIAK

IPC分类号： C12P21/00 ,  C12N1/20

CPC分类号： C12P21/00 ,  C12N1/20

摘要： A method of reducing precipitation in a bacterial fermentation process for producing a fermentation product, which fermentation process comprises the steps:

a) cultivating the host cells for bacterial growth in a batch phase using a batch medium; and
b) cultivating the host cells for producing the fermentation product in a fed-batch phase using a feed medium;

which feed and batch media are each unprecipitated media solutions, wherein:

i) the batch medium comprises an amount of calcium salts, magnesium salts, phosphate salts and at least one chelating agent, which is a citrate salt and/or citric acid at an amount of at least 5 mM, and/or EDTA at an amount of at least 1 mM; and
ii) the feed medium comprises an amount of calcium salts which is at least 1 mM, and which is at least 5-fold higher as compared to the batch medium (M/M); and
iii) the feed medium comprises an amount of magnesium salts which is at least 3 mM, and which is at least 2-fold higher as compared to the batch medium (M/M); and
iv) the feed medium comprises an amount of phosphate salts which is less than 90% of the amount comprised in the batch medium (M/M).