公开(公告)号：US20130011882A1

公开(公告)日：2013-01-10

申请号：US13472058

申请日：2012-05-15

申请人： LUIS G. CASCÃO-PEREIRA ,  JAMES T. KELLIS, JR. ,  BRADLEY A. PAULSON ,  SCOTT D. POWER ,  SANDRA W. RAMER ,  VIVEK SHARMA ,  ANDREW SHAW ,  JAYARAMA K. SHETTY ,  DONALD E. WARD

发明人： LUIS G. CASCÃO-PEREIRA ,  JAMES T. KELLIS, JR. ,  BRADLEY A. PAULSON ,  SCOTT D. POWER ,  SANDRA W. RAMER ,  VIVEK SHARMA ,  ANDREW SHAW ,  JAYARAMA K. SHETTY ,  DONALD E. WARD

IPC分类号： C12N9/28 ,  C12N9/16 ,  C12G3/00 ,  C12P19/14 ,  C12S9/00 ,  A23L1/09 ,  C12N15/63 ,  C12N1/21

CPC分类号： C11D3/38681 ,  C11D3/386 ,  C12N9/2417 ,  D06M16/003

摘要： Disclosed are compositions comprising variants of alpha-amylase that have alpha-amylase activity and which exhibit altered properties relative to a parent AmyS-like alpha-amylase from which they are derived. The compositions comprise an additional enzyme such as a phytase. Also disclosed are methods of using the compositions, and kits related thereto.

公开(公告)号：US20110091958A1

公开(公告)日：2011-04-21

申请号：US12776921

申请日：2010-05-10

申请人： AYROOKARAN J. POULOSE ,  DAVID A. ESTELL ,  JAMES T. KELLIS, JR. ,  RICHARD R. BOTT

发明人： AYROOKARAN J. POULOSE ,  DAVID A. ESTELL ,  JAMES T. KELLIS, JR. ,  RICHARD R. BOTT

IPC分类号： C12N9/48 ,  C07H21/04 ,  C12N15/63 ,  C12N1/21

CPC分类号： C12N9/54 ,  C11D3/386 ,  C11D3/38609 ,  C12Y304/21062

摘要： Novel enzyme variants including protease variants derived from the DNA sequences of naturally-occurring or recombinant non-human proteases are disclosed. The variant proteases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant protease to generate the substitution of a plurality of amino acid residues in the amino acid sequence of a precursor protease. Such variant proteases have properties which are different from those of the precursor protease, such as altered wash performance. The substituted amino acid residue correspond to positions 27, 45, 170, 181, 251 and 271 of Bacillus amyloliquefaciens subtilisin. Additional variants comprising at least one additional substituion at a positon selected from 1, 14, 49, 61, 87, 100, 102, 118, 128, 204 and 258 of Bacillus amyloliquefaciens subtilisin are also described.

摘要翻译： 公开了包含衍生自天然或重组非人蛋白酶的DNA序列的蛋白酶变体的新型酶变体。 一般来说，变体蛋白酶通过体外修饰编码天然存在或重组蛋白酶的前体DNA序列获得，以产生前体蛋白酶的氨基酸序列中的多个氨基酸残基的取代。 这种变体蛋白酶具有与前体蛋白酶不同的性质，例如改变的洗涤性能。 取代的氨基酸残基对应于解淀粉芽孢杆菌枯草杆菌蛋白酶的位置27,45,170,181,251和271。 还描述了在解淀粉芽孢杆菌枯草杆菌蛋白酶的1,4,14,69,61,87,100,102,118,128,204和258的选择位置中至少包含一个另外的取代基的另外的变体。