公开(公告)号：US10011861B2

公开(公告)日：2018-07-03

申请号：US14826518

申请日：2015-08-14

Applicant: LUMINEX CORPORATION

Inventor： Martin Kochanczyk ,  Doug Whitman

IPC: C12Q1/68 ,  C12Q1/6827

CPC classification number: C12Q1/6827 ,  C12Q2521/327 ,  C12Q2525/121 ,  C12Q2525/131 ,  C12Q2525/301

Abstract: Nucleic acid constructs and methods that provide superior prevention of primer-dimers and other artifacts of false priming events are disclosed. In particular, there is disclosed a hairpin primer having a target-specific primer region, wherein the target-specific region comprises a target-binding dependent cleavage sequence; a first stem forming region 5′ of the target-specific primer region; and a second stem forming region 3′ of the target-specific primer region, wherein the second stem forming region is complementary to the first stem forming region. Methods of using the hairpin primer to amplify a target nucleic acid are also disclosed.

公开(公告)号：US11371082B2

公开(公告)日：2022-06-28

申请号：US16869758

申请日：2020-05-08

Applicant: LUMINEX CORPORATION

Inventor： Martin Kochanczyk ,  Doug Whitman

IPC: C12Q1/6827

Abstract: Nucleic acid constructs and methods that provide superior prevention of primer-dimers and other artifacts of false priming events are disclosed. In particular, there is disclosed a hairpin primer having a target-specific primer region, wherein the target-specific region comprises a target-binding dependent cleavage sequence; a first stem forming region 5′ of the target-specific primer region; and a second stem forming region 3′ of the target-specific primer region, wherein the second stem forming region is complementary to the first stem forming region. Methods of using the hairpin primer to amplify a target nucleic acid are also disclosed.

公开(公告)号：US10683535B2

公开(公告)日：2020-06-16

申请号：US16001201

申请日：2018-06-06

Applicant: LUMINEX CORPORATION

Inventor： Martin Kochanczyk ,  Doug Whitman

IPC: C12Q1/68 ,  C12Q1/6827

Abstract: Nucleic acid constructs and methods that provide superior prevention of primer-dimers and other artifacts of false priming events are disclosed. In particular, there is disclosed a hairpin primer having a target-specific primer region, wherein the target-specific region comprises a target-binding dependent cleavage sequence; a first stem forming region 5′ of the target-specific primer region; and a second stem forming region 3′ of the target-specific primer region, wherein the second stem forming region is complementary to the first stem forming region. Methods of using the hairpin primer to amplify a target nucleic acid are also disclosed.