公开(公告)号：US10011861B2

公开(公告)日：2018-07-03

申请号：US14826518

申请日：2015-08-14

Applicant: LUMINEX CORPORATION

Inventor： Martin Kochanczyk ,  Doug Whitman

IPC: C12Q1/68 ,  C12Q1/6827

CPC classification number: C12Q1/6827 ,  C12Q2521/327 ,  C12Q2525/121 ,  C12Q2525/131 ,  C12Q2525/301

Abstract: Nucleic acid constructs and methods that provide superior prevention of primer-dimers and other artifacts of false priming events are disclosed. In particular, there is disclosed a hairpin primer having a target-specific primer region, wherein the target-specific region comprises a target-binding dependent cleavage sequence; a first stem forming region 5′ of the target-specific primer region; and a second stem forming region 3′ of the target-specific primer region, wherein the second stem forming region is complementary to the first stem forming region. Methods of using the hairpin primer to amplify a target nucleic acid are also disclosed.

公开(公告)号：US11261481B2

公开(公告)日：2022-03-01

申请号：US16804060

申请日：2020-02-28

Applicant: LUMINEX CORPORATION

Inventor： Doug Whitman ,  Nicolas Arab ,  Chuck Collins

IPC: C12Q1/68 ,  C07H21/00 ,  C12Q1/6818

Abstract: Methods and compositions for the detection and quantification of nucleic acids are provided. In certain embodiments, methods involve the use of primers or probes that comprise a non-natural nucleotide linked to a reporter. Target nucleic acids are detected by the polymerization of a complementary probe or primer that incorporated a cognate non-natural nucleotide linked to a quencher.

公开(公告)号：US10179899B2

公开(公告)日：2019-01-15

申请号：US14731459

申请日：2015-06-05

Applicant: LUMINEX CORPORATION

Inventor： Doug Whitman ,  James Heisler ,  Colin Bozarth ,  Germaine Siew ,  Charles Collins ,  Eric Smith

IPC: B01L7/00 ,  C12M1/06 ,  B01F13/08

Abstract: Methods and systems for magnetic mixing. Particular embodiments relate to applying a magnetic field to move a magnetically responsive component in a chamber.

公开(公告)号：US10851336B2

公开(公告)日：2020-12-01

申请号：US16202315

申请日：2018-11-28

Applicant: LUMINEX CORPORATION

Inventor： Doug Whitman ,  James Heisler ,  Colin Bozarth ,  Germaine Siew ,  Charles Collins ,  Eric Smith

IPC: B01L7/00 ,  C12M1/06 ,  B01F13/08 ,  B01F13/00 ,  B01F13/10

Abstract: Methods and systems for magnetic mixing. Particular embodiments relate to applying a magnetic field to move a magnetically responsive component in a chamber.

公开(公告)号：US10683535B2

公开(公告)日：2020-06-16

申请号：US16001201

申请日：2018-06-06

Applicant: LUMINEX CORPORATION

Inventor： Martin Kochanczyk ,  Doug Whitman

IPC: C12Q1/68 ,  C12Q1/6827

Abstract: Nucleic acid constructs and methods that provide superior prevention of primer-dimers and other artifacts of false priming events are disclosed. In particular, there is disclosed a hairpin primer having a target-specific primer region, wherein the target-specific region comprises a target-binding dependent cleavage sequence; a first stem forming region 5′ of the target-specific primer region; and a second stem forming region 3′ of the target-specific primer region, wherein the second stem forming region is complementary to the first stem forming region. Methods of using the hairpin primer to amplify a target nucleic acid are also disclosed.

公开(公告)号：US09982291B2

公开(公告)日：2018-05-29

申请号：US14823288

申请日：2015-08-11

Applicant: LUMINEX CORPORATION

Inventor： Scott C. Johnson ,  Nicolas Arab ,  Doug Whitman

IPC: C12Q1/68 ,  C07H21/00 ,  C12P19/34

CPC classification number: C12Q1/6818 ,  C12Q1/6816 ,  C12Q1/6823 ,  C12Q2521/301 ,  C12Q2521/327 ,  C12Q2525/101 ,  C12Q2525/121 ,  C12Q2525/161 ,  C12Q2525/186 ,  C12Q2525/301 ,  C12Q2527/107 ,  C12Q2537/143 ,  C12Q2561/113 ,  C12Q2565/101

Abstract: Methods and compositions for the detection and quantification of nucleic acids are provided. In certain embodiments, methods involve the use of cleavable probes that comprise a ribonucleotide position that is susceptible to endoribonuclease (e.g., RNase H) cleavage in the presence of target nucleic acid molecules. Probes of the embodiments may also comprise non-natural nucleotide linked to a reporter and/or quenching moiety.

公开(公告)号：US11371082B2

公开(公告)日：2022-06-28

申请号：US16869758

申请日：2020-05-08

Applicant: LUMINEX CORPORATION

Inventor： Martin Kochanczyk ,  Doug Whitman

IPC: C12Q1/6827

Abstract: Nucleic acid constructs and methods that provide superior prevention of primer-dimers and other artifacts of false priming events are disclosed. In particular, there is disclosed a hairpin primer having a target-specific primer region, wherein the target-specific region comprises a target-binding dependent cleavage sequence; a first stem forming region 5′ of the target-specific primer region; and a second stem forming region 3′ of the target-specific primer region, wherein the second stem forming region is complementary to the first stem forming region. Methods of using the hairpin primer to amplify a target nucleic acid are also disclosed.

公开(公告)号：US11371080B2

公开(公告)日：2022-06-28

申请号：US16690525

申请日：2019-11-21

Applicant: LUMINEX CORPORATION

Inventor： Doug Whitman

IPC: C12Q1/6876 ,  C12Q1/6818

Abstract: Methods and compositions for the detection and quantification of nucleic acids are provided. In certain embodiments, methods involve the use of cleavable probes capable of forming double-stranded structures, such as hairpin structures, which probes can be distinguished from one another on the basis of reporter signal, melt properties, or both.

公开(公告)号：US10975419B2

公开(公告)日：2021-04-13

申请号：US15962077

申请日：2018-04-25

Applicant: LUMINEX CORPORATION

Inventor： Scott C. Johnson ,  Nicolas Arab ,  Doug Whitman

IPC: C12Q1/6818 ,  C07H21/00 ,  C12Q1/6816 ,  C12Q1/6823

Abstract: Methods and compositions for the detection and quantification of nucleic acids are provided. In certain embodiments, methods involve the use of cleavable probes that comprise a ribonucleotide position that is susceptible to endoribonuclease (e.g., RNase H) cleavage in the presence of target nucleic acid molecules. Probes of the embodiments may also comprise non-natural nucleotide linked to a reporter and/or quenching moiety.

公开(公告)号：US10752939B2

公开(公告)日：2020-08-25

申请号：US15656541

申请日：2017-07-21

Applicant: LUMINEX CORPORATION

Inventor： Scott C. Johnson ,  Nicolas Arab ,  Doug Whitman

IPC: C12Q1/68 ,  C07H21/00 ,  C12Q1/6818 ,  C12Q1/6816 ,  C12Q1/6823

Abstract: Methods and compositions for the detection and quantification of nucleic acids are provided. In certain embodiments, methods involve the use of cleavable probes that comprise a ribonucleotide position that is susceptible to endoribonuclease (e.g., RNase H) cleavage in the presence of target nucleic acid molecules. Probes of the embodiments may also comprise non-natural nucleotide linked to a reporter and/or quenching moiety.