公开(公告)号：US20060292647A1

公开(公告)日：2006-12-28

申请号：US11069351

申请日：2005-03-01

申请人： Lawrence Green ,  Anne Sherwood

发明人： Lawrence Green ,  Anne Sherwood

IPC分类号： G01N33/554 ,  G01N33/569

CPC分类号： G01N33/5695 ,  G01N33/56911

摘要： The present disclosure concerns methods, compositions and apparatus for detecting pathogens and/or molecular markers. In a particular embodiment, the pathogen to be detected may be Mycobacterium bovis or any other Mycobacterium species that causes tuberculosis in a mammal. However, the disclosed methods are not limited and virtually any type of pathogen and/or molecular marker may be screened and detected. Preferred embodiments comprise reflex supplemental testing using the same assay at approximately 100% sensitivity and the highest possible corresponding sensitivity—in one example 70%. Such assay conditions, used iteratively, result in elimination of 70% of uninfected subjects for each round of testing. Use of 4 or more rounds of testing results in less than 1% error. Since only positive samples are retested, the methods provide a rapid, inexpensive and highly accurate way to detect infected subjects.

摘要翻译： 本公开涉及用于检测病原体和/或分子标记物的方法，组合物和装置。 在一个具体实施方案中，待检测的病原体可以是牛分枝杆菌或在哺乳动物中导致结核病的任何其它分枝杆菌属物种。 然而，所公开的方法不受限制，并且实际上可以筛选和检测任何类型的病原体和/或分子标记。 优选的实施方案包括使用相同测定法的反射补充测试，其在约100％的灵敏度和最高可能的相应灵敏度下（在一个实施例中为70％）。 反复使用的这种测定条件导致每轮测试中70％的未感染受试者的消除。 使用4轮或更多轮测试会导致小于1％的误差。 由于只有阳性样本被重新测试，所以这些方法提供了快速，廉价和高度准确的方式来检测感染的受试者。

公开(公告)号：US20070238651A1

公开(公告)日：2007-10-11

申请号：US11215356

申请日：2005-08-29

申请人： Eric Holmes ,  Anne Sherwood

发明人： Eric Holmes ,  Anne Sherwood

IPC分类号： A61K48/00 ,  A61K38/16 ,  C12P21/06 ,  C12P19/04 ,  C12N9/10

CPC分类号： C12N9/1051 ,  A61K38/00 ,  A61K39/00 ,  C12N9/1077 ,  C12P19/18 ,  C12P19/26

摘要： A rat ganglioside GM1-specific α1→2fucosyltransferase is disclosed. Nucleotide sequences of a rat ganglioside GM1-specific α1→2fucosyltransferase, amino acid sequences of its encoded protein (including peptide or polypeptide), and derivatives thereof are described. Also described are fragments (and derivatives and analogs thereof) which comprise a domain of rat ganglioside GM1-specific α1→2fucosyltransferase with catalytic activity. Methods of production of rat ganglioside GM1-specific α1→2fucosyltransferase and derivatives and analogs thereof (e.g. by recombinant means) are provided. Methods of inhibiting the function of rat ganglioside GM1-specific α1→2fucosyltransferase (e.g. by means of antisense RNA) are provided. Methods of commercial scale use of the rat ganglioside GM1-specific α1→2fucosyltransferase in the production of fucosyl-saccharide compositions are described. Applications of these compositions, e.g. as additives for human nutritive compositions or immunotherapeutics for cancer, are disclosed.

摘要翻译： 公开了一种大鼠神经节苷脂GM1特异性α1-2岩藻糖基转移酶。 描述了大鼠神经节苷脂GM1特异性α1-2岩藻糖基转移酶的核苷酸序列，其编码的蛋白质（包括肽或多肽）的氨基酸序列及其衍生物。 还描述了包含具有催化活性的大鼠神经节苷脂GM1特异性α1-2岩藻糖基转移酶的结构域的片段（及其衍生物和类似物）。 提供了大鼠神经节苷脂GM1特异性α1-2岩藻糖基转移酶及其衍生物和类似物的生产方法（例如通过重组方法）。 提供了抑制大鼠神经节苷脂GM 1 - 特异性α1-2岩藻糖基转移酶（例如通过反义RNA）的功能的方法。 描述了岩藻糖基糖组合物生产中商业规模使用大鼠神经节苷脂GM 1特异性α1-> 2岩藻糖基转移酶的方法。 这些组合物的应用，例如 作为人类营养组合物或癌症免疫治疗剂的添加剂。