公开(公告)号：US06210897B1

公开(公告)日：2001-04-03

申请号：US09393554

申请日：1999-09-10

申请人： Leif Andersson ,  James Kijas ,  Sophie Gafvert ,  Gunilla Wigh-Trowaldh ,  Ake Hedhammar

发明人： Leif Andersson ,  James Kijas ,  Sophie Gafvert ,  Gunilla Wigh-Trowaldh ,  Ake Hedhammar

IPC分类号： C12Q168

CPC分类号： C07K14/70553 ,  C12Q1/6883 ,  C12Q2600/124 ,  C12Q2600/156

摘要： The present invention relates to an isolated nucleic acid molecule encoding canine leukocyte integrin &bgr;-2 subunit having a Cys36Ser missense mutation, wherein the missense mutation is indicative of a carrier of canine leukocyte adhesion deficiency. The present invention also relates to a method for identifying dogs which are carriers of or are affected with canine leukocyte adhesion deficiency. This method includes obtaining a biological sample from a dog and testing the biological sample for a Cys36Ser missense mutation in a gene encoding leukocyte integrin &bgr;-2 subunit, wherein the missense mutation in one allele is indicative of a carrier of canine leukocyte adhesion deficiency and the missense mutation in both alleles is indicative of a dog affected with canine leukocyte adhesion deficiency.

摘要翻译： 本发明涉及编码具有Cys36Ser错义突变的犬白细胞整合素β-2亚基的分离的核酸分子，其中所述错义突变指示犬白细胞粘附缺陷的载体。 本发明还涉及用于鉴定作为犬白细胞粘附缺陷的载体或受其影响的狗的方法。 该方法包括从狗获得生物样品，并测试生物样品中编码白细胞整合素β-2亚基的基因中的Cys36Ser错义突变，其中一个等位基因中的错义突变指示犬白细胞粘附缺陷的载体， 两个等位基因中的错义突变表明狗受到白细胞粘附不足的影响。