公开(公告)号：US06287841B1

公开(公告)日：2001-09-11

申请号：US08090207

申请日：1993-07-27

申请人： Leonardus Johannes Sofie Marie Mulleners ,  Onno Misset ,  Jan Metske Van Der Laan ,  Franciscus Josephus Cornelius Van Gastel ,  Cornelis Petrus Broekhuizen ,  Erik Jan Baas

发明人： Leonardus Johannes Sofie Marie Mulleners ,  Onno Misset ,  Jan Metske Van Der Laan ,  Franciscus Josephus Cornelius Van Gastel ,  Cornelis Petrus Broekhuizen ,  Erik Jan Baas

IPC分类号： C12N954

CPC分类号： C11D3/386 ,  C12N9/54

摘要： New PB92 or Subtilisin 309 mutant serine proteases are provided having specific mutations, resulting in an surprisingly better wash performance or in an improved storage stability with at similar or even better wash performance. These PB92 or Subtilisin 309 mutants include mutations at positions 60, 87, 97, 99, 102, 116, 117, 126, 127, 128, 130, 133, 134, 154, 156, 158, 159, 160, 164, 169, 175, 180, 182, 193, 197, 198, 203, 211, and 216. The new proteases, therefore, are very suitable for use in various types of detergents, whether or not in conjunction with other enzymes, for example amylases, cellulases and lipases. Preferred embodiments are the PB92 and Subtilisin 309 mutants having a mutation at position 102 and in particular those having at least one further mutation.

摘要翻译： 提供新的PB92或枯草杆菌蛋白酶309突变丝氨酸蛋白酶具有特异性突变，导致惊人的更好的洗涤性能或改善的储存稳定性，同样或甚至更好的洗涤性能。 这些PB92或枯草杆菌蛋白酶309突变体包括位置60,87,97,99,102,116,117,126,127,128,130,133,134,154,156,158,159,160,164,169的突变， 因此，新的蛋白酶非常适合用于各种类型的洗涤剂，无论是否与其它酶联合使用，例如淀粉酶，纤维素酶 和脂肪酶。 优选的实施方案是在102位具有突变的PB92和枯草杆菌蛋白酶309突变体，特别是具有至少一个其它突变的突变体。