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    Proteases from gram-positive organisms
    1.
    发明授权
    Proteases from gram-positive organisms 有权
    来自革兰氏阳性菌的蛋白酶

    公开(公告)号：US06300117B1

    公开(公告)日：2001-10-09

    申请号：US09308375

    申请日：1999-05-14

    申请人： David A. Estell

    发明人： David A. Estell

    IPC分类号： C12N954

    CPC分类号： C12N9/54 ,  A23K20/189 ,  C11D3/386 ,  C12N15/75 ,  D06M16/003

    摘要： The present invention relates to the identification of novel metallo-proteases (MP) in Gram-positive microorganisms. The present invention provides the nucleic acid and amino acid sequences for Bacillus (MP). The present invention also provides host cells having mutation or deletion of part or all of the gene encoding MP. The present invention also provides host cells further comprising nucleic acid encoding desired heterologous proteins such as enzymes. The present invention also provides cleaning compositions comprising an MP of the present invention.

    摘要翻译： 本发明涉及革兰阳性微生物中新型金属蛋白酶（MP）的鉴定。 本发明提供芽孢杆菌（MP）的核酸和氨基酸序列。 本发明还提供具有编码MP的基因的部分或全部的突变或缺失的宿主细胞。 本发明还提供宿主细胞，其进一步包含编码所需异源蛋白质如酶的核酸。 本发明还提供了包含本发明的MP的清洁组合物。

    High alkaline serine protease
    2.
    发明授权
    High alkaline serine protease 失效
    高碱性丝氨酸蛋白酶

    公开(公告)号：US06287841B1

    公开(公告)日：2001-09-11

    申请号：US08090207

    申请日：1993-07-27

    申请人： Leonardus Johannes Sofie Marie Mulleners ,  Onno Misset ,  Jan Metske Van Der Laan ,  Franciscus Josephus Cornelius Van Gastel ,  Cornelis Petrus Broekhuizen ,  Erik Jan Baas

    发明人： Leonardus Johannes Sofie Marie Mulleners ,  Onno Misset ,  Jan Metske Van Der Laan ,  Franciscus Josephus Cornelius Van Gastel ,  Cornelis Petrus Broekhuizen ,  Erik Jan Baas

    IPC分类号： C12N954

    CPC分类号： C11D3/386 ,  C12N9/54

    摘要： New PB92 or Subtilisin 309 mutant serine proteases are provided having specific mutations, resulting in an surprisingly better wash performance or in an improved storage stability with at similar or even better wash performance. These PB92 or Subtilisin 309 mutants include mutations at positions 60, 87, 97, 99, 102, 116, 117, 126, 127, 128, 130, 133, 134, 154, 156, 158, 159, 160, 164, 169, 175, 180, 182, 193, 197, 198, 203, 211, and 216. The new proteases, therefore, are very suitable for use in various types of detergents, whether or not in conjunction with other enzymes, for example amylases, cellulases and lipases. Preferred embodiments are the PB92 and Subtilisin 309 mutants having a mutation at position 102 and in particular those having at least one further mutation.

    摘要翻译： 提供新的PB92或枯草杆菌蛋白酶309突变丝氨酸蛋白酶具有特异性突变，导致惊人的更好的洗涤性能或改善的储存稳定性，同样或甚至更好的洗涤性能。 这些PB92或枯草杆菌蛋白酶309突变体包括位置60,87,97,99,102,116,117,126,127,128,130,133,134,154,156,158,159,160,164,169的突变， 因此，新的蛋白酶非常适合用于各种类型的洗涤剂，无论是否与其它酶联合使用，例如淀粉酶，纤维素酶 和脂肪酶。 优选的实施方案是在102位具有突变的PB92和枯草杆菌蛋白酶309突变体，特别是具有至少一个其它突变的突变体。

    Subtilisin protease variants having amino acid substitutions in defined epitope regions
    3.
    发明授权
    Subtilisin protease variants having amino acid substitutions in defined epitope regions 失效
    枯草杆菌蛋白酶变体在定义的表位区域具有氨基酸取代

    公开(公告)号：US06586223B1

    公开(公告)日：2003-07-01

    申请号：US09618573

    申请日：2000-07-18

    申请人： Elizabeth Ellen Sikorski ,  Donn Nelton Rubingh

    发明人： Elizabeth Ellen Sikorski ,  Donn Nelton Rubingh

    IPC分类号： C12N954

    CPC分类号： C12N9/54 ,  C11D3/386

    摘要： The present invention relates to variants of subtilisin-like proteases having decreased immunogenicity relative to their corresponding wild-type proteases. More particularly, the present invention relates to variants having a modified amino acid sequence of a wild-type amino acid sequence, wherein the modified amino acid sequence comprises a substitution of one or more epitope regions. The invention further relates to mutant genes encoding such variants and cleaning and personal care compositions comprising such variants.

    摘要翻译： 本发明涉及相对于其相应的野生型蛋白酶具有降低的免疫原性的枯草杆菌蛋白酶样蛋白酶的变体。 更具体地，本发明涉及具有野生型氨基酸序列的修饰的氨基酸序列的变体，其中修饰的氨基酸序列包含一个或多个表位区域的取代。 本发明还涉及编码这种变体的突变基因和包含这些变体的清洁和个人护理组合物。

    Subtilase variants having an improved wash performance on egg stains
    6.
    发明授权
    Subtilase variants having an improved wash performance on egg stains 有权
    具有改善的蛋渍洗涤性能的脱丝酶变体

    公开(公告)号：US06727085B2

    公开(公告)日：2004-04-27

    申请号：US09736116

    申请日：2000-12-13

    申请人： Tina Sejersgård Fanø ,  Frank Mikkelsen

    发明人： Tina Sejersgård Fanø ,  Frank Mikkelsen

    IPC分类号： C12N954

    CPC分类号： C12N9/54 ,  C11D3/38636

    摘要： The present invention relates to the use of a subtilase variant for removal of egg stains from laundry or from hard surfaces, where the subtilase variant comprises at least one additional amino acid residue in the active site loop (b) region from position 95 to 103 (BASBPN numbering). These subtilase variants are useful exhibiting excellent or improved wash performance on egg stains when used in e.g. cleaning or detergent compositions, including automatic dishwash compositions. The present invention also relates to novel subtilase variants, to isolated DNA sequences encoding the variants, expression vectors, host cells, and methods for producing and using the variants of the invention. Further, the present invention relates to cleaning and detergent compositions comprising the variants of the invention.

    摘要翻译： 本发明涉及用于从衣物或硬质表面除去蛋渍的枯草芽孢杆菌变体的用途，其中所述枯草芽孢杆菌变体在95至103位的活性位点环（b）区域中包含至少一个另外的氨基酸残基（ BASBPN编号）。 这些枯草芽孢杆菌变体在用于例如蛋白质时在蛋渍上表现出优异或改善的洗涤性能是有用的。 清洁或洗涤剂组合物，包括自动洗碗组合物。 本发明还涉及编码变体，表达载体，宿主细胞的分离的DNA序列以及用于生产和使用本发明的变体的方法的新型脱羧酶变体。 此外，本发明涉及包含本发明变体的清洁和洗涤剂组合物。

    Multiply-substituted protease variants with altered net charge for use in detergents
    7.
    发明授权
    Multiply-substituted protease variants with altered net charge for use in detergents 有权

    公开(公告)号：US06673590B1

    公开(公告)日：2004-01-06

    申请号：US09177353

    申请日：1998-10-23

    申请人： Ayrookaran .J. Poulose ,  Volker Schellenberger ,  James T. Kellis, Jr. ,  Christian Paech ,  Joanne Nadherny ,  Donald P. Naki ,  Katherine D. Collier ,  Robert M. Caldwell

    发明人： Ayrookaran .J. Poulose ,  Volker Schellenberger ,  James T. Kellis, Jr. ,  Christian Paech ,  Joanne Nadherny ,  Donald P. Naki ,  Katherine D. Collier ,  Robert M. Caldwell

    IPC分类号： C12N954

    CPC分类号： C12N9/54 ,  A23G4/123 ,  A23K20/189 ,  A61K8/66 ,  A61K2800/86 ,  A61Q11/00 ,  A61Q19/00 ,  C11D3/386 ,  C11D3/38609 ,  C11D3/38618 ,  C11D3/3907 ,  C11D3/3945 ,  C12Y304/00 ,  C12Y304/21062

    摘要： Novel protease variants derived from the DNA sequences of naturally-occurring or recombinant non-human proteases are disclosed. The variant proteases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant protease to generate the substitution of a plurality of amino acid residues in the amino acid sequence of a precursor protease. Protease variants are provided that contain substitutions of the amino acids at one or more residue positions so that the substitution alters the charge at that position to make the charge more negative or less positive compared to a precursor protease and thus the protease variant is more effective in a low detergent concentration system than a precursor protease. Also provided are protease variants containing substitutions of the amino acids at one or more residue positions so that the substitution alters the charge at that position to make the charge more positive or less negative compared to a precursor protease and thus the protease variant is more effective in a high detergent concentration system than a precursor protease. Protease variants are provided that contain substitutions of the amino acids at one or more residue positions so that the substitution alters the charge at that position to make the charge more negative or less positive compared to a precursor protease and thus the protease variant is more effective in a medium detergent concentration system than a precursor protease. Also provided are protease variants containing substitutions of the amino acids at one or more residue positions so that the substitution alters the charge at that position to make the charge more positive or less negative compared to a precursor protease and thus the protease variant is more effective in a medium detergent concentration system than a precursor protease. Further provided is a method of producing a protease variant that is more effective in a low detergent concentration system, medium detergent concentration system and high detergent concentration system than a precursor protease.

    Subtilisin protease variants having amino acid deletions and substitutions in defined epitope regions
    8.
    发明授权
    Subtilisin protease variants having amino acid deletions and substitutions in defined epitope regions 失效
    枯草杆菌蛋白酶变体在确定的表位区域具有氨基酸缺失和取代

    公开(公告)号：US06586224B1

    公开(公告)日：2003-07-01

    申请号：US09618845

    申请日：2000-07-18

    申请人： Donn Nelton Rubingh ,  Elizabeth Ellen Sikorski

    发明人： Donn Nelton Rubingh ,  Elizabeth Ellen Sikorski

    IPC分类号： C12N954

    CPC分类号： C12N9/54 ,  C11D3/386

    摘要： The present invention relates to variants of subtilisin-like proteases having decreased immunogenicity relative to their corresponding wild-type proteases. The present invention further relates to such variants additionally having one or more amino acid substitutions in one or more epitope regions or additionally having one or more stabilizing substitutions. The invention further relates to mutant genes encoding such variants and cleaning and personal care compositions comprising such variants.

    摘要翻译： 本发明涉及相对于其相应的野生型蛋白酶具有降低的免疫原性的枯草杆菌蛋白酶样蛋白酶的变体。 本发明还涉及另外在一个或多个表位区域中另外具有一个或多个氨基酸取代或另外具有一个或多个稳定取代的这些变体。 本发明还涉及编码这种变体的突变基因和包含这些变体的清洁和个人护理组合物。

    Subtilase variants
    9.
    发明授权
    Subtilase variants 失效
    Subtilase变体

    公开(公告)号：US06190900B1

    公开(公告)日：2001-02-20

    申请号：US09120577

    申请日：1998-07-22

    申请人： Laurens Nicolaas Sierkstra ,  Jan Klugkist ,  Peter Markvardsen ,  Claus von der Osten ,  Peter Bauditz

    发明人： Laurens Nicolaas Sierkstra ,  Jan Klugkist ,  Peter Markvardsen ,  Claus von der Osten ,  Peter Bauditz

    IPC分类号： C12N954

    CPC分类号： C11D3/386 ,  C12N9/54

    摘要： The present invention relates to enzymes produced by mutating the genes for a number of subtilases and expressing the mutated genes in suitable hosts are presented. The enzymes exhibit improved stability and/or improved wash performance in any detergent in comparison to their wild type parent enzymes. The enzymes are well-suited for use in any detergent and for some in especially liquid or solid shaped detergent compositions.

    摘要翻译： 本发明涉及通过突变多个枯草芽孢杆菌基因并在合适的宿主中表达突变的基因而产生的酶。 与其野生型亲本酶相比，酶在任何洗涤剂中表现出改进的稳定性和/或改善的洗涤性能。 这些酶非常适合用于任何洗涤剂和一些特别是液体或固体形式的洗涤剂组合物中。