公开(公告)号：US20080318250A1

公开(公告)日：2008-12-25

申请号：US10588445

申请日：2005-02-07

申请人： Linda Gilmer ,  Arabinda Mandal ,  Michael J. Wolkowicz ,  Kenneth L. Klotz ,  John C. Herr

发明人： Linda Gilmer ,  Arabinda Mandal ,  Michael J. Wolkowicz ,  Kenneth L. Klotz ,  John C. Herr

IPC分类号： G01N33/53 ,  C07H1/00 ,  C12P19/34

CPC分类号： C07K16/28 ,  G01N33/56966

摘要： Methods and compositions for identifying and isolating sperm cells from samples containing multiple cell types are described. The methods and compositions employ antibodies that specifically bind to sperm-specific antigens located on or internal to the sperm plasma membrane. A reporter molecule may be conjugated to the antibodies to aid in the detection of sperm. The antibodies may be targeted to sperm-specific antigens in the head and/or tail of sperm to facilitate the identification and isolation of sperm cells from forensic samples prepared from sexual assault evidence. Purified DNA from the isolated sperm cells can be amplified by polymerase chain reaction to assist forensic analysis in sexual assault cases.

摘要翻译： 描述了从含有多种细胞类型的样品中鉴定和分离精子细胞的方法和组合物。 方法和组合物使用特异性结合位于精子质膜上或内部的精子特异性抗原的抗体。 报告分子可以与抗体缀合以帮助检测精子。 抗体可以针对精子头部和/或尾部的精子特异性抗原，以促进从性攻击证据准备的法医样品中鉴定和分离精子细胞。 可以通过聚合酶链反应扩增分离的精子细胞的纯化DNA，以协助性攻击病例的法医分析。