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公开(公告)号：US20030049708A1
公开(公告)日：2003-03-13
申请号：US10139740
申请日：2002-05-07
IPC分类号： C12Q001/42 , C12Q001/04
CPC分类号： C12Q1/04 , C12Q1/008 , C12Q1/66 , G01N2333/30
摘要： Micro-organisms which over-produce ATPase, especially mycoplasmas in a sample of a cell culture, are detected by making use of the ATPase activity to convert cellular or externally added ATP to ADP. The decrease in ATP externally added or the increase or absolute level of ADP produced from cellular ATP can then be detected or measured. For example, if a reagent such as phosphenol pyruvate/pyruvate kinase is added to convert that ADP back to ATP, the resulting high level of ATP can be detected bioluminescently using the reaction: Luciferinnullluciferase enzymenullATPnullnullnullproducts.
摘要翻译： 通过利用ATPase活性将细胞或外部添加的ATP转化成ADP来检测过量产生ATP酶的微生物，特别是细胞培养物样品中的支原体。 然后可以检测或测量外部添加的ATP的减少或由细胞ATP产生的ADP的增加或绝对水平。 例如，如果加入诸如磷酸酚丙酮酸/丙酮酸激酶的试剂以将该ADP转化回ATP，则可以使用荧光素+荧光素酶+ ATP-> nu +产物的反应来生物发光检测所得到的高水平ATP。
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公开(公告)号：US20030008338A1
公开(公告)日：2003-01-09
申请号：US10005189
申请日：2001-12-07
IPC分类号： C12Q001/66 , C12Q001/48
CPC分类号： C12Q1/66 , C12Q1/485 , G01N33/5014
摘要： The invention relates to methods for detecting the cytotoxic activity of an effector, such as a cytotoxic T lymphocyte, on a non-microbial target cell. Detection of cytotoxic activity is preferably achieved by detecting adenylate kinase release using photometric methods.
摘要翻译： 本发明涉及用于检测效应物如细胞毒性T淋巴细胞在非微生物靶细胞上的细胞毒活性的方法。 细胞毒活性的检测优选通过使用光度法检测腺苷酸激酶释放来实现。
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公开(公告)号：US20040253658A1
公开(公告)日：2004-12-16
申请号：US10892285
申请日：2004-07-16
IPC分类号： C12Q001/68 , C12Q001/48
CPC分类号： C12Q1/485 , G01N2500/04
摘要： Methods and kits for detecting kinase activity A method for measuring protein kinase activity comprising: (a) providing a first solution comprising ATP and a protein kinase to be tested, and a second solution comprising ATP in the absence of said kinase to be tested; (b) adding a substrate capable of being phosphorylated by the protein kinase to be tested to the first and second solutions of step (a); (c) measuring the concentration of ATP and/or ADP, or the rate of change thereof with respect to time, in each of the reaction mixtures formed in step (b) using a bioluminescence reaction; and (d) using the information about the concentration of ATP and/or ADP to determine the activity of the protein kinase to be tested.
摘要翻译： 用于检测激酶活性的方法和试剂盒用于测量蛋白激酶活性的方法，包括：（a）提供包含ATP和待测试的蛋白激酶的第一溶液，以及在不存在所述待测试激酶的情况下包含ATP的第二溶液; （b）将待测试的蛋白激酶磷酸化的底物加入到步骤（a）的第一和第二溶液中; （c）使用生物发光反应测量在步骤（b）中形成的每种反应混合物中ATP和/或ADP的浓度或其相对于时间的变化速率; 和（d）使用关于ATP和/或ADP的浓度的信息来确定待测试的蛋白激酶的活性。
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公开(公告)号：US20040077030A1
公开(公告)日：2004-04-22
申请号：US10426973
申请日：2003-05-01
IPC分类号： C12Q001/48
CPC分类号： C12Q1/485 , G01N2500/04
摘要： A method for measuring protein kinase activity comprising: (a) providing a first solution comprising ATP and a protein kinase to be tested, and a second solution comprising ATP in the absence of said kinase to be tested; (b) adding a substrate capable of being phosphorylated by the protein kinase to be tested to the first and second solutions of step (a); (c) measuring the concentration of ATP and/or ADP, or the rate of change thereof with respect to time, in each of the reaction mixtures formed in step (b) using a bioluminescence reaction; and (d) using the information about the concentration of ATP and/or ADP to determine the activity of the protein kinase to be tested.
摘要翻译： 一种测定蛋白激酶活性的方法，包括：（a）提供包含待测试的ATP和蛋白激酶的第一溶液，以及在不存在所述待测试激酶的情况下包含ATP的第二溶液; （b）将待测试的蛋白激酶磷酸化的底物加入到步骤（a）的第一和第二溶液中; （c）使用生物发光反应测量在步骤（b）中形成的每种反应混合物中ATP和/或ADP的浓度或其相对于时间的变化速率; 和（d）使用关于ATP和/或ADP的浓度的信息来确定待测试的蛋白激酶的活性。
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公开(公告)号：US20020172991A1
公开(公告)日：2002-11-21
申请号：US10014816
申请日：2001-12-14
IPC分类号： C12Q001/48
CPC分类号： C12Q1/485 , G01N2500/04
摘要： A method for measuring protein kinase activity comprising: (a) providing a first solution comprising ATP and a protein kinase to be tested, and a second solution comprising ATP in the absence of said kinase to be tested; (b) adding a substrate capable of being phosphorylated by the protein kinase to be tested to the first and second solutions of step (a); (c) measuring the concentration of ATP and/or ADP, or the rate of change thereof with respect to time, in each of the reaction mixtures formed in step (b) using a bioluminescence reaction; and (d) using the information about the concentration of ATP and/or ADP to determine the activity of the protein kinase to be tested.
摘要翻译： 一种测定蛋白激酶活性的方法，包括：（a）提供包含待测试的ATP和蛋白激酶的第一溶液，以及在不存在所述待测试激酶的情况下包含ATP的第二溶液; （b）将待测试的蛋白激酶磷酸化的底物加入到步骤（a）的第一和第二溶液中; （c）使用生物发光反应测量在步骤（b）中形成的每种反应混合物中ATP和/或ADP的浓度或其相对于时间的变化速率; 和（d）使用关于ATP和/或ADP的浓度的信息来确定待测试的蛋白激酶的活性。
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