公开(公告)号：US20080118472A1

公开(公告)日：2008-05-22

申请号：US11295853

申请日：2005-12-06

Applicant: Lyle M. Rode ,  C. James Newbold

Inventor： Lyle M. Rode ,  C. James Newbold

IPC: A61K35/74 ,  C12N1/21 ,  C12P13/08

CPC classification number: C12Q1/04 ,  A23K10/18 ,  A23K20/142 ,  A23K50/10 ,  G01N2333/34

Abstract: Methods for increasing the resistance to rumen inactivation of a cultured Gram positive bacteria strain useful for gastrointestinal delivery of bioactive compounds to ruminants, which includes the steps of growing a culture of the bacteria strain through at least one passage in a growth medium containing an amount of lysozyme effective to induce the growth of bacterial cell walls resistant to protozoal predation; and recovering the bacteria strain from the lysozyme-containing medium. Rumen-bypass feed supplements produced by the inventive method are also disclosed, as well as methods for supplementing the diet of a ruminant with the rumen bypass feed supplements and an in vitro method for evaluating the resistance of Gram positive bacteria strains to rumen inactivation in vivo.

Abstract translation: 用于增加对用于向反刍动物胃肠道递送生物活性化合物的培养的革兰氏阳性菌菌株对瘤胃灭活的抗性的方法，其包括通过生长培养基中培养细菌菌株的培养物的步骤，所述生长培养基含有一定量的 有效诱导抗原生动物捕食的细菌细胞壁生长的溶菌酶; 并从含溶菌酶的培养基中回收细菌菌株。 还公开了通过本发明方法产生的瘤胃旁路饲料补充剂以及用瘤胃旁路饲料补充剂补充反刍动物饮食的方法和用于评估革兰氏阳性菌菌株对体内瘤胃失活的抗性的体外方法 。