公开(公告)号：US20210130812A1

公开(公告)日：2021-05-06

申请号：US16830565

申请日：2020-03-26

申请人： MACROGEN, INC. ,  PSOMAGEN, INC.

发明人： JOSHUA SUNGWOO YANG ,  JAEKYUNG CHON ,  IK JUNG CHOI ,  HYUN MIN PARK ,  JIEUN PARK ,  JEONGSUN SEO ,  CHANGHOON KIM ,  JONG YEON SHIN ,  HAN SOL SEO ,  JIWON SHIN ,  IN HEE HWANG ,  SEON HYE SIM ,  CHANG WOO CHO ,  KYUIN HWANG ,  IN SEON KIM ,  HYUNG IL LEE ,  JUNG HYUN CHO

IPC分类号： C12N15/10 ,  C12Q1/6806 ,  C12Q1/6844

摘要： Disclosed are a method for DNA extraction in a sample for next generation sequencing (NGS) and a method of constructing a NGS library using the extracted DNA. The method for DNA extraction includes: preparing a mixture by mixing a biological sample with a buffer; applying microwaves to the mixture; and recovering DNA. The method of constructing a NGS library includes: extracting DNA according to the method for DNA extraction; amplifying a target DNA using primers; and purifying the amplified product and subjecting the purified product to library pooling.