公开(公告)号：US20150064138A1

公开(公告)日：2015-03-05

申请号：US14475785

申请日：2014-09-03

Applicant: MASSACHUSETTS INSTITUTE OF TECHNOLOGY

Inventor： TIMOTHY KUAN-TA LU ,  ROBERT JAMES CITORIK ,  MARK KYLE MIMEE

IPC: C12N9/22

Abstract: Various aspects and embodiments of the invention are directed to methods and compositions for reversing antibiotic resistance or virulence in and/or destroying pathogenic microbial cells such as, for example, pathogenic bacterial cells. The methods include exposing microbial cells to a delivery vehicle with at least one nucleic acid encoding an engineered autonomously distributed circuit that contains a programmable nuclease targeted to one or multiple genes of interest.

Abstract translation: 本发明的各个方面和实施方案涉及用于逆转抗生素抗性或在其中和/或破坏致病微生物细胞例如致病细菌细胞的方法和组合物。 所述方法包括将微生物细胞暴露于具有编码设计的自主分布电路的至少一种核酸的递送载体，所述核酸包含靶向一个或多个感兴趣的基因的可编程核酸酶。